Detailed information of TA system TA242531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3447..4117
• Replicon: plasmid p34Pae8-KPC
• Accession: NZ_CP095775
• Organism: Pseudomonas aeruginosa strain 34Pae8

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: MX625_RS32120
• Protein ID: WP_058565749.1
• Coordinates: 3680..4117 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: MX625_RS32115
• Protein ID: WP_058565750.1
• Coordinates: 3447..3683 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MX625_RS32095	1..1155	+	1155	WP_229602000.1	replication initiator protein A	-
MX625_RS32100	1702..2352	+	651	WP_229602001.1	DNA-binding protein	-
MX625_RS32105	2380..2685	+	306	WP_229602002.1	hypothetical protein	-
MX625_RS32110	3012..3437	+	426	WP_229602003.1	DUF192 domain-containing protein	-
MX625_RS32115	3447..3683	+	237	WP_058565750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MX625_RS32120	3680..4117	+	438	WP_058565749.1	PIN domain-containing protein	Toxin
MX625_RS32125	4124..4660	+	537	WP_058565748.1	hypothetical protein	-
MX625_RS32130	4676..5488	+	813	WP_058565747.1	3'-5' exonuclease	-
MX625_RS32135	5478..5786	+	309	WP_058565746.1	hypothetical protein	-
MX625_RS32140	5798..6058	+	261	WP_058565745.1	hypothetical protein	-
MX625_RS32145	6739..7575	+	837	WP_058565766.1	DUF932 domain-containing protein	-
MX625_RS32150	7631..8059	+	429	WP_058565765.1	hypothetical protein	-
MX625_RS32155	8075..8578	+	504	WP_229602004.1	hypothetical protein	-
MX625_RS32160	8639..8989	+	351	WP_015272403.1	TrfB-related DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / aac(6')-29b / aac(3)-Id	-	1..42729	42729

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15723.11 Da
• Isoelectric Point: 8.5199

>T242531 WP_058565749.1 NZ_CP095775:3680-4117 [Pseudomonas aeruginosa]
MSKKTYMLDTNICSFIMRERPDAVLAMLEKAVSSQHRIVVSAITYSEMRFGAANPKASPKVATMVDAFVQRLDAVLPWDT
AAVDQTTQIRTTLARLGTPIGNNDAAIAGHALAAGCVLVTNNTREFARVPGLVLEDWTQPTTTKS

Nucleotide

• Length: 438 bp

>T242531 NZ_OW706648:4564872-4565156 [Salmonella enterica subsp. enterica serovar Typhi]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9012.22 Da
• Isoelectric Point: 4.4091

>AT242531 WP_058565750.1 NZ_CP095775:3447-3683 [Pseudomonas aeruginosa]
MRTVSIFMNCRNQAIRIPRDMEYQGVSELEIIRDGETLVLRPVRPSWGSLRDVAPADPDFLTDRQDVIEEGRFLGDFT

Nucleotide

• Length: 237 bp

>AT242531 NZ_OW706648:4564640-4564882 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA