Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 3447..4117 Replicon plasmid p34Pae8-KPC
Accession NZ_CP095775
Organism Pseudomonas aeruginosa strain 34Pae8

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag MX625_RS32120 Protein ID WP_058565749.1
Coordinates 3680..4117 (+) Length 146 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID -
Locus tag MX625_RS32115 Protein ID WP_058565750.1
Coordinates 3447..3683 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MX625_RS32095 1..1155 + 1155 WP_229602000.1 replication initiator protein A -
MX625_RS32100 1702..2352 + 651 WP_229602001.1 DNA-binding protein -
MX625_RS32105 2380..2685 + 306 WP_229602002.1 hypothetical protein -
MX625_RS32110 3012..3437 + 426 WP_229602003.1 DUF192 domain-containing protein -
MX625_RS32115 3447..3683 + 237 WP_058565750.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MX625_RS32120 3680..4117 + 438 WP_058565749.1 PIN domain-containing protein Toxin
MX625_RS32125 4124..4660 + 537 WP_058565748.1 hypothetical protein -
MX625_RS32130 4676..5488 + 813 WP_058565747.1 3'-5' exonuclease -
MX625_RS32135 5478..5786 + 309 WP_058565746.1 hypothetical protein -
MX625_RS32140 5798..6058 + 261 WP_058565745.1 hypothetical protein -
MX625_RS32145 6739..7575 + 837 WP_058565766.1 DUF932 domain-containing protein -
MX625_RS32150 7631..8059 + 429 WP_058565765.1 hypothetical protein -
MX625_RS32155 8075..8578 + 504 WP_229602004.1 hypothetical protein -
MX625_RS32160 8639..8989 + 351 WP_015272403.1 TrfB-related DNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 / aac(6')-29b / aac(3)-Id - 1..42729 42729


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15723.11 Da        Isoelectric Point: 8.5199

>T242531 WP_058565749.1 NZ_CP095775:3680-4117 [Pseudomonas aeruginosa]
MSKKTYMLDTNICSFIMRERPDAVLAMLEKAVSSQHRIVVSAITYSEMRFGAANPKASPKVATMVDAFVQRLDAVLPWDT
AAVDQTTQIRTTLARLGTPIGNNDAAIAGHALAAGCVLVTNNTREFARVPGLVLEDWTQPTTTKS

Download         Length: 438 bp

>T242531 NZ_OW706648:4564872-4565156 [Salmonella enterica subsp. enterica serovar Typhi]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 9012.22 Da        Isoelectric Point: 4.4091

>AT242531 WP_058565750.1 NZ_CP095775:3447-3683 [Pseudomonas aeruginosa]
MRTVSIFMNCRNQAIRIPRDMEYQGVSELEIIRDGETLVLRPVRPSWGSLRDVAPADPDFLTDRQDVIEEGRFLGDFT

Download         Length: 237 bp

>AT242531 NZ_OW706648:4564640-4564882 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References