Detailed information of TA system TA242526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 990159..990745
• Replicon: chromosome
• Accession: NZ_CP095774
• Organism: Pseudomonas aeruginosa strain 34Pae8

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: MX625_RS04690
• Protein ID: WP_003120987.1
• Coordinates: 990159..990458 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: MX625_RS04695
• Protein ID: WP_003448662.1
• Coordinates: 990455..990745 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MX625_RS04670	985272..985805	-	534	WP_003149521.1	type IV pilus biogenesis protein PilP	-
MX625_RS04675	985795..987120	-	1326	WP_022579625.1	type 4b pilus protein PilO2	-
MX625_RS04680	987124..988833	-	1710	WP_023093673.1	PilN family type IVB pilus formation outer membrane protein	-
MX625_RS04685	988833..989957	-	1125	WP_023093672.1	TcpQ domain-containing protein	-
MX625_RS04690	990159..990458	+	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MX625_RS04695	990455..990745	+	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
MX625_RS04700	990828..991163	-	336	WP_022579623.1	hypothetical protein	-
MX625_RS04705	991302..993278	-	1977	WP_023093671.1	DEAD/DEAH box helicase	-
MX625_RS04710	993275..995170	-	1896	WP_155713823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	933056..1022738	89682

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T242526 WP_003120987.1 NZ_CP095774:990159-990458 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T242526 NZ_OW706648:c3623365-3622991 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGTCAAAGGGTCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGTCGAATTGAAGCGAAACATGCGCT
GGAAGCGTATCCGCCACAAAATGCGATTAGCCTTATCACATGGATGGAAGTGATGGTGGGTGCTAAGAAATATCATCAGG
AAAACCGCACGCGCATTGCATTAAGTGCGTTCAATATTATTGGCGTAACACAGGAAATCGCGGAACGAAGCGTCATTGTC
AGGCAGGAGTATGGCATGAAACTCCCGGATGCGATCATTCTGGCCACTGCACAAGTCCATCGTTGTGAGCTTGTGACGCG
CAATACAAAAGACTTTGCCGATATTCCGGGAGTGATCACGCCGTATCACTTGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT242526 WP_003448662.1 NZ_CP095774:990455-990745 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT242526 NZ_OW706648:c3623607-3623365 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGATGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTACCTCGTGCAGAACTCTTGCGGGAGGCCGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAGACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGCGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Structures

Toxin

• AlphaFold DB: G8CP73