Detailed information of TA system TA242521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 5219320..5220578
• Replicon: chromosome
• Accession: NZ_CP095770
• Organism: Pseudomonas aeruginosa strain 34Pae36

Toxin (Protein)

• Gene name: -
• Uniprot ID: V6AJ34
• Locus tag: MX654_RS25070
• Protein ID: WP_003086331.1
• Coordinates: 5219320..5220075 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: MX654_RS25075
• Protein ID: WP_003086329.1
• Coordinates: 5220210..5220578 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MX654_RS25060	5214491..5217034	+	2544	WP_033955245.1	acyl-homoserine-lactone acylase QuiP	-
MX654_RS25065	5217190..5218551	-	1362	WP_003120632.1	DNA recombination protein RmuC	-
MX654_RS25070	5219320..5220075	-	756	WP_003086331.1	RES family NAD+ phosphorylase	Toxin
MX654_RS25075	5220210..5220578	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
MX654_RS25080	5220651..5221937	-	1287	WP_003086327.1	FAD-binding oxidoreductase	-
MX654_RS25085	5221993..5223486	-	1494	WP_015648322.1	aldehyde dehydrogenase family protein	-
MX654_RS25090	5223661..5224137	+	477	WP_004353334.1	elongation factor GreAB	-
MX654_RS25095	5224140..5224415	-	276	WP_023088486.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28236.34 Da
• Isoelectric Point: 7.7294

>T242521 WP_003086331.1 NZ_CP095770:c5220075-5219320 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T242521 NZ_OW706648:1027706-1027808 [Salmonella enterica subsp. enterica serovar Typhi]
GCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT242521 WP_003086329.1 NZ_CP095770:c5220578-5220210 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT242521 NZ_OW706648:c1027810-1027667 [Salmonella enterica subsp. enterica serovar Typhi]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCTCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Structures

Toxin

• AlphaFold DB: V6AJ34

Antitoxin

• AlphaFold DB: V6AJU7