Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 253540..254182 Replicon chromosome
Accession NZ_CP095767
Organism Bacillus cereus strain CDHWZ7

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag MYW48_RS01400 Protein ID WP_000635965.1
Coordinates 253832..254182 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag MYW48_RS01395 Protein ID WP_000004570.1
Coordinates 253540..253827 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYW48_RS01370 (MYW48_01370) 248857..249819 + 963 WP_000961146.1 UV DNA damage repair endonuclease UvsE -
MYW48_RS01375 (MYW48_01375) 249812..250384 - 573 WP_000908529.1 rhomboid family intramembrane serine protease -
MYW48_RS01380 (MYW48_01380) 250478..250837 + 360 WP_000583418.1 holo-ACP synthase -
MYW48_RS01385 (MYW48_01385) 250994..251944 + 951 WP_002084009.1 outer membrane lipoprotein carrier protein LolA -
MYW48_RS01390 (MYW48_01390) 252062..253231 + 1170 WP_000390595.1 alanine racemase -
MYW48_RS01395 (MYW48_01395) 253540..253827 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
MYW48_RS01400 (MYW48_01400) 253832..254182 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
MYW48_RS01405 (MYW48_01405) 254250..256418 + 2169 WP_000426210.1 Tex family protein -
MYW48_RS01410 (MYW48_01410) 256476..256592 - 117 WP_001143640.1 cortex morphogenetic protein CmpA -
MYW48_RS01415 (MYW48_01415) 256788..257246 + 459 WP_000343552.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T242514 WP_000635965.1 NZ_CP095767:253832-254182 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T242514 NZ_OW706512:c2995077-2994703 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGTCAAAGGGTCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGTCGAATTGAAGCGAAACATGCGCT
GGAAGCGTATCCGCCACAAAATGCGATTAGCCTTATCACATGGATGGAAGTGATGGTGGGTGCTAAGAAATATCATCAGG
AAAACCGCACGCGCATTGCATTAAGTGCGTTCAATATTATTGGCGTAACACAGGAAATCGCGGAACGAAGCGTCATTGTC
AGGCAGGAGTATGGCATGAAACTCCCGGATGCGATCATTCTGGCCACTGCACAAGTCCATCGTTGTGAGCTTGTGACGCG
CAATACAAAAGACTTTGCCGATATTCCGGGAGTGATCACGCCGTATCACTTGTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT242514 WP_000004570.1 NZ_CP095767:253540-253827 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT242514 NZ_OW706512:c2995319-2995077 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGATGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTACCTCGTGCAGAACTCTTGCGGGAGGCCGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAGACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGCGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References