Detailed information of TA system TA024250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 649478..650109
• Accession: NC_013205
• Organism: Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C8WSZ4
• Locus tag: AACI_RS03060
• Protein ID: WP_012810012.1
• Coordinates: 649702..650109 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C8WSZ3
• Locus tag: AACI_RS03055
• Protein ID: WP_012810011.1
• Coordinates: 649478..649705 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AACI_RS03025	644914..645699	+	786	WP_012810006.1	SAF domain-containing protein	-
AACI_RS03030	645683..646951	+	1269	WP_012810007.1	CpaF family protein	-
AACI_RS03035	646948..647814	+	867	WP_012810008.1	hypothetical protein	-
AACI_RS03040	647814..648620	+	807	WP_012810009.1	type II secretion system F family protein	-
AACI_RS16290	648672..648953	-	282	WP_041707288.1	hypothetical protein	-
AACI_RS03050	648952..649380	+	429	WP_041707291.1	hypothetical protein	-
AACI_RS03055	649478..649705	+	228	WP_012810011.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AACI_RS03060	649702..650109	+	408	WP_012810012.1	type II toxin-antitoxin system VapC family toxin	Toxin
AACI_RS03065	650151..650747	+	597	WP_012810013.1	hypothetical protein	-
AACI_RS03070	650761..651204	+	444	WP_012810014.1	A24 family peptidase	-
AACI_RS03075	651201..651647	+	447	WP_012810015.1	hypothetical protein	-
AACI_RS03080	651672..653411	+	1740	WP_012810016.1	hypothetical protein	-
AACI_RS03090	654775..654981	+	207	WP_012810019.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	648970..660737	11767

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (7-48)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15503.93 Da
• Isoelectric Point: 9.5158

>T24250 WP_012810012.1 NC_013205:649702-650109 [Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446]
MRYLLDTNMCIYLINRRNPALIERFRQYRTGDIGVSVITVAELMYGIAKSRHPERNRAALSVFLLPLEIAPFDERAAFHY
GRIRAQLESEGQPIGAMDTLIAAHALSLDVTVVTRNVREFQRVRGLRVENWFEGV

Nucleotide

• Length: 408 bp

>T24250 NC_013205:649702-650109 [Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446]
ATGAGGTACCTCCTGGACACCAACATGTGCATTTATCTCATCAATCGCCGAAATCCAGCACTCATTGAACGGTTTCGGCA
ATATCGCACAGGCGACATTGGCGTCTCCGTGATCACGGTTGCGGAGTTGATGTACGGCATAGCGAAGAGTCGGCATCCGG
AACGAAATCGAGCGGCTTTGTCCGTGTTTCTCTTGCCACTTGAGATCGCGCCATTTGACGAGCGCGCGGCATTTCACTAC
GGGCGGATCCGAGCGCAGTTGGAATCCGAAGGTCAGCCCATTGGCGCTATGGATACCTTGATTGCCGCGCATGCCTTGTC
GCTCGACGTGACGGTGGTCACGCGCAACGTGCGGGAGTTTCAGCGTGTTCGAGGATTGCGAGTCGAAAACTGGTTTGAAG
GCGTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8516.71 Da
• Isoelectric Point: 4.3322

>AT24250 WP_012810011.1 NC_013205:649478-649705 [Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446]
VEKAKLFQNGSSQAVRLPKAYRFDGDEVYVKRIGEAVVLLPMDGAWDTMAEALRMFSEDLVLERDEPLTDVRESL

Nucleotide

• Length: 228 bp

>AT24250 NC_013205:649478-649705 [Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446]
GTGGAGAAAGCGAAGTTGTTTCAAAACGGAAGCAGCCAAGCGGTAAGGTTGCCAAAGGCGTATCGGTTTGACGGAGACGA
AGTGTACGTCAAGCGTATTGGTGAGGCGGTCGTGCTTCTGCCAATGGATGGCGCATGGGACACCATGGCTGAGGCGCTTC
GCATGTTTTCGGAGGATTTGGTGCTTGAGCGGGACGAACCGCTGACGGATGTGCGGGAGAGTTTATGA

Structures

Toxin

• AlphaFold DB: C8WSZ4

Antitoxin

• AlphaFold DB: C8WSZ3