Detailed information of TA system TA242499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 78811..79333
• Replicon: plasmid pKP5362-2
• Accession: NZ_CP095764
• Organism: Klebsiella pneumoniae strain KP5362

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: MYA94_RS27265
• Protein ID: WP_004187019.1
• Coordinates: 79049..79333 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: MYA94_RS27260
• Protein ID: WP_004187025.1
• Coordinates: 78811..79059 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYA94_RS27230 (MYA94_27230)	76178..76747	-	570	WP_004152117.1	small heat shock protein sHSP20	-
MYA94_RS27235 (MYA94_27235)	76782..77063	-	282	WP_013307885.1	helix-turn-helix domain-containing protein	-
MYA94_RS27240 (MYA94_27240)	77307..77570	-	264	WP_004118208.1	hypothetical protein	-
MYA94_RS27245 (MYA94_27245)	77585..77848	+	264	WP_004187027.1	transposase	-
MYA94_RS27250 (MYA94_27250)	77793..77909	+	117	Protein_80	transposase domain-containing protein	-
MYA94_RS27255 (MYA94_27255)	78097..78521	+	425	Protein_81	IS1 family transposase	-
MYA94_RS27260 (MYA94_27260)	78811..79059	+	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
MYA94_RS27265 (MYA94_27265)	79049..79333	+	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MYA94_RS27270 (MYA94_27270)	79349..80704	-	1356	WP_004187017.1	ISNCY family transposase	-
MYA94_RS27275 (MYA94_27275)	80786..81037	-	252	Protein_85	conjugal transfer protein TraH	-
MYA94_RS27280 (MYA94_27280)	81694..82107	-	414	WP_004187013.1	hypothetical protein	-
MYA94_RS27285 (MYA94_27285)	83650..84114	+	465	WP_004187010.1	conjugative transfer protein MobI(A/C)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkA / mrkB / mrkC / mrkF / mrkJ / mrkJ	1..128470	128470
	-	flank	IS/Tn	-	-	78148..78372	224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T242499 WP_004187019.1 NZ_CP095764:79049-79333 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T242499 NZ_OW704627:c361273-360872 [Salmonella enterica subsp. enterica serovar Typhi]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCCTTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGACAG
GACTGGACGCTCTTGCGTCCTCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
AG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT242499 WP_004187025.1 NZ_CP095764:78811-79059 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT242499 NZ_OW704627:c361520-361254 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4