Detailed information of TA system TA242498

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 65179..65915
• Replicon: plasmid pKP5362-2
• Accession: NZ_CP095764
• Organism: Klebsiella pneumoniae strain KP5362

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: MYA94_RS27170
• Protein ID: WP_004187044.1
• Coordinates: 65179..65661 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: MYA94_RS27175
• Protein ID: WP_003026799.1
• Coordinates: 65649..65915 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYA94_RS27145 (MYA94_27145)	61203..62139	-	937	Protein_59	ISNCY family transposase	-
MYA94_RS27150 (MYA94_27150)	62249..62398	+	150	Protein_60	transposase	-
MYA94_RS27155 (MYA94_27155)	62414..62524	+	111	Protein_61	IS3 family transposase	-
MYA94_RS27160 (MYA94_27160)	62758..63462	-	705	WP_031591821.1	toll/interleukin-1 receptor domain-containing protein	-
MYA94_RS27165 (MYA94_27165)	63622..64968	-	1347	WP_077251107.1	ISNCY family transposase	-
MYA94_RS27170 (MYA94_27170)	65179..65661	-	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
MYA94_RS27175 (MYA94_27175)	65649..65915	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
MYA94_RS27180 (MYA94_27180)	66066..66769	+	704	Protein_66	IS6-like element IS26 family transposase	-
MYA94_RS27185 (MYA94_27185)	66928..69513	-	2586	WP_004187040.1	EAL domain-containing protein	-
MYA94_RS27190 (MYA94_27190)	69482..69727	-	246	WP_032238678.1	hypothetical protein	-
MYA94_RS27195 (MYA94_27195)	69785..70767	-	983	Protein_69	IS5-like element ISKpn26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkA / mrkB / mrkC / mrkF / mrkJ / mrkJ	1..128470	128470
	-	inside	IScluster/Tn	-	-	61182..70767	9585

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T242498 WP_004187044.1 NZ_CP095764:c65661-65179 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T242498 NZ_OW704627:c344528-344130 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCTGAAATTCATGCTTGATACCAATACTTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCACCGG
AGCGTAATTTTGCAGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTACCGAACTGGCCCGCAAGGGAACACCTGTCGGGTCTTATGACCAGATGATTGCTGGCCATGCCCGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAGTTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT242498 WP_003026799.1 NZ_CP095764:c65915-65649 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT242498 NZ_OW704627:c344755-344528 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGCACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66