Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4626890..4627700 Replicon chromosome
Accession NZ_CP095762
Organism Klebsiella pneumoniae strain KP5362

Toxin (Protein)


Gene name KacT Uniprot ID A0A483G813
Locus tag MYA94_RS22715 Protein ID WP_023279404.1
Coordinates 4626890..4627423 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag MYA94_RS22720 Protein ID WP_002887278.1
Coordinates 4627434..4627700 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MYA94_RS22710 (4625721) 4625721..4626842 + 1122 WP_002887282.1 cupin domain-containing protein -
MYA94_RS22715 (4626890) 4626890..4627423 - 534 WP_023279404.1 type II toxin-antitoxin system toxin KacT Toxin
MYA94_RS22720 (4627434) 4627434..4627700 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
MYA94_RS22725 (4627803) 4627803..4629236 - 1434 WP_004192214.1 Cu(+)/Ag(+) sensor histidine kinase -
MYA94_RS22730 (4629226) 4629226..4629909 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
MYA94_RS22735 (4630081) 4630081..4631466 + 1386 WP_004192218.1 efflux transporter outer membrane subunit -
MYA94_RS22740 (4631484) 4631484..4631828 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19809.71 Da        Isoelectric Point: 6.2369

>T242492 WP_023279404.1 NZ_CP095762:c4627423-4626890 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T242492 NZ_OW704291:c3666877-3666389 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATCTCCGCCCCTGAACCGCTTCATGCCGGGCATATTCTTACCCCCTTCTGCTGCGGTGTGGATTCAATGGATAACTG
GCTAAAACAGCGGGCGATGAAAAATCAGGTAACTGGCGCATCCCGTACCTTTGTGTGTTGCGGCAGCGATTCTGACGTAC
TGGCGTATTATTCGCTGGCGTCCAGCGCGGTAACGACAAACACCGCCCCCGGTCGCTTTCGTCGCAATATGCCCGATCCG
ATCCCGGTTGTGGTGCTGGGCCGTCTGGCGGTGGATCGGTCGCTACATGGGCAGGGCGTAGGTCGGGCGCTGGTGAGGGA
TGCCGGGCTGCGGGTGATTCAGGTGGCGGAAACTATCGGTATTCGCGGGATGCTGGTCCATGCCCTGTCGGATGAAGCGC
GGGAGTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTT
CATGCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT242492 WP_002887278.1 NZ_CP095762:c4627700-4627434 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT242492 NZ_OW704291:c3667167-3666874 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCCAGTCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGCATCAAGCCCGCCGAGCGCGATCTTATCGA
TCGGGCTGCAAAAGCCAGAGGGAAAAACTGCACAGACTTTGTGCTAGAAGCTGCCCGCGCCGCCGCCGAGGAAGCGCTGA
TCGAGCAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGCATTTCTGATCCGTCTGGATCAGGCTCCTGCGCCAAAC
GATGCGTTGCGTAAAACCATGCAGACTCCTGCACCGTGGGAGCAGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483G813

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References