Detailed information of TA system TA024249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 416251..416854
• Accession: NC_013205
• Organism: Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: F8ICH1
• Locus tag: AACI_RS01905
• Protein ID: WP_008336350.1
• Coordinates: 416504..416854 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AACI_RS01900
• Protein ID: WP_012809791.1
• Coordinates: 416251..416499 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AACI_RS01875	411316..411663	-	348	WP_041707806.1	phage holin family protein	-
AACI_RS01880	411811..412218	+	408	WP_148213718.1	holo-ACP synthase	-
AACI_RS01885	412301..413803	+	1503	WP_012809788.1	bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase	-
AACI_RS01890	413813..414844	+	1032	WP_012809789.1	outer membrane lipoprotein carrier protein LolA	-
AACI_RS01895	414938..416140	+	1203	WP_012809790.1	alanine racemase	-
AACI_RS01900	416251..416499	+	249	WP_012809791.1	hypothetical protein	Antitoxin
AACI_RS01905	416504..416854	+	351	WP_008336350.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AACI_RS01910	416946..418199	+	1254	WP_012809792.1	adenosylhomocysteinase	-
AACI_RS01915	418223..418984	+	762	WP_012809793.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
AACI_RS01920	418924..420480	-	1557	WP_012809794.1	HD domain-containing protein	-
AACI_RS01925	420704..421021	+	318	WP_012809795.1	DUF1659 domain-containing protein	-
AACI_RS01930	421084..421302	+	219	WP_012809796.1	DUF2922 domain-containing protein	-
AACI_RS01935	421347..421613	-	267	WP_012809797.1	YuzB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12898.95 Da
• Isoelectric Point: 5.7235

>T24249 WP_008336350.1 NC_013205:416504-416854 [Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446]
MNVRRGDIFFADLSPVVGSEQGGFRPVLVVQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIKAEKYGLERDSVILLEQL
RTIDKQRLTDKITHLDDEAMKLVNEGLLISLGLVDF

Nucleotide

• Length: 351 bp

>T24249 NC_013205:416504-416854 [Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446]
TTGAATGTCAGGCGTGGGGACATCTTCTTTGCGGATCTGTCGCCCGTGGTCGGCTCGGAGCAAGGAGGATTTCGCCCCGT
ACTCGTGGTGCAAAACGACATCGGCAACCGCTTCAGCCCGACGGTGATTGTGGCGGCCATCACCGCGCAGATCCAGAAGG
CCAAGTTGCCGACGCACGTGGAGATCAAGGCCGAGAAGTACGGTCTGGAGCGCGATTCCGTGATTCTGCTCGAGCAGCTG
CGCACCATCGATAAGCAGCGCCTGACCGACAAGATCACGCATCTGGACGACGAAGCGATGAAGCTCGTCAACGAGGGGCT
GTTGATCAGCCTCGGCCTGGTGGATTTCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9304.50 Da
• Isoelectric Point: 7.9798

>AT24249 WP_012809791.1 NC_013205:416251-416499 [Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446]
MSSSKFNAWNQAAGRESGDKRARDIVREALRMYVSRREPGDLRETMQRGYQEMAPLNLRIACEAFLVEQEAGNTVERLVS
GV

Nucleotide

• Length: 249 bp

>AT24249 NC_013205:416251-416499 [Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446]
TTGTCGAGTTCGAAATTCAACGCCTGGAATCAAGCCGCGGGTCGCGAATCCGGAGACAAACGCGCGCGTGACATCGTGCG
CGAGGCGCTGCGTATGTATGTATCGAGGAGAGAGCCCGGAGATCTGCGCGAGACCATGCAGCGCGGGTATCAGGAGATGG
CGCCTCTCAACCTGCGCATCGCCTGTGAGGCGTTTCTGGTCGAGCAGGAGGCCGGCAACACCGTGGAGCGCTTGGTGAGC
GGGGTGTGA

Structures

Toxin

• AlphaFold DB: F8ICH1