Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2079321..2079911 Replicon chromosome
Accession NZ_CP095761
Organism Klebsiella michiganensis strain K254

Toxin (Protein)


Gene name pemK Uniprot ID J5V7J4
Locus tag MMY93_RS09985 Protein ID WP_009653321.1
Coordinates 2079579..2079911 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag MMY93_RS09980 Protein ID WP_004852307.1
Coordinates 2079321..2079578 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MMY93_RS09960 (MMY93_09960) 2075219..2075824 - 606 WP_227635132.1 glutathione S-transferase family protein -
MMY93_RS09965 (MMY93_09965) 2076004..2076930 + 927 WP_049078228.1 LysR substrate-binding domain-containing protein -
MMY93_RS09970 (MMY93_09970) 2076968..2077966 - 999 WP_049078227.1 aldo/keto reductase -
MMY93_RS09975 (MMY93_09975) 2078067..2078981 + 915 WP_086531142.1 LysR family transcriptional regulator -
MMY93_RS09980 (MMY93_09980) 2079321..2079578 + 258 WP_004852307.1 antitoxin Antitoxin
MMY93_RS09985 (MMY93_09985) 2079579..2079911 + 333 WP_009653321.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MMY93_RS09995 (MMY93_09995) 2080234..2081691 + 1458 WP_032743138.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
MMY93_RS10005 (MMY93_10005) 2082654..2084108 - 1455 WP_197664188.1 AMP nucleosidase -
MMY93_RS10010 (MMY93_10010) 2084240..2084497 - 258 WP_009653336.1 histidine kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2070256..2079911 9655


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11823.69 Da        Isoelectric Point: 10.5834

>T242466 WP_009653321.1 NZ_CP095761:2079579-2079911 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGIKTTGVIRCDQPRTI
DIAARNGKRLERIPDAVVNDVLARLDAIFS

Download         Length: 333 bp

>T242466 NZ_OW485601:c3049736-3049470 [Rhodovastum atsumiense]
TTGAAGGTCCATTTCAGTGAGGAAGGCTGGGAGGATTACCAGCACTGGTGCCAGCACGACCGGACCGTGCTCGACAAGAT
CAACGCCCTTATCGAGGACACAAGGCGGCGGCCTTTCACCGGCCTCGGCAAACCGGAACCGCTGAAAGCCGCGCTATCGG
GGTGGTGGTCCCGCCGCATCACCGGCGAGCATCGGCTCGTCTACCGCGTTGCCGGGGCCGGCGCCGAACAGCGCATCGAA
ATCGTCCAGTGCCGGTACCACTATTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT242466 WP_004852307.1 NZ_CP095761:2079321-2079578 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT242466 NZ_OW485601:c3050005-3049733 [Rhodovastum atsumiense]
ATGCCCCATGTGCCCTATACCGAGCTGAGGCAGAACCTGTCCCGCTACATGGACGAGGCAATCGAAAGCCGCGTGCCCAT
CACGATCACCCGGCAGGGCGGCAAGGGCAACGTGGTGCTGCTGTCGGAAGAAGAGTTCGAGGGGTGGATGGAGACGGTCC
ACCTGCTCCGCAGCCCCGCCAATGCCCAGCGGCTGCTGCGGTCCATCGCTTCCGTCGAGGCCGGGGGCGCCCGGGAGCGG
GACATCATCCCCGCCACCGGACCACGGGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J5V7J4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References