Detailed information of TA system TA242464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1223110..1224027
• Replicon: chromosome
• Accession: NZ_CP095760
• Organism: Bacillus velezensis strain ZK-3

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: MXH98_RS06465
• Protein ID: WP_012117368.1
• Coordinates: 1223281..1224027 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: MXH98_RS06460
• Protein ID: WP_003154807.1
• Coordinates: 1223110..1223280 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MXH98_RS06410 (MXH98_06410)	1218244..1218756	+	513	WP_012117362.1	sigma-70 family RNA polymerase sigma factor	-
MXH98_RS06415 (MXH98_06415)	1218869..1219102	+	234	WP_012117363.1	XtmA	-
MXH98_RS06420 (MXH98_06420)	1219118..1219966	+	849	WP_012117364.1	hypothetical protein	-
MXH98_RS06425 (MXH98_06425)	1219979..1220350	+	372	WP_012117365.1	XkdW family protein	-
MXH98_RS06430 (MXH98_06430)	1220355..1220552	+	198	WP_012117366.1	XkdX family protein	-
MXH98_RS06435 (MXH98_06435)	1220609..1221370	+	762	WP_012117367.1	hypothetical protein	-
MXH98_RS06440 (MXH98_06440)	1221422..1221685	+	264	WP_003154815.1	hemolysin XhlA family protein	-
MXH98_RS06445 (MXH98_06445)	1221699..1221962	+	264	WP_003154813.1	phage holin	-
MXH98_RS06450 (MXH98_06450)	1221976..1222854	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
MXH98_RS06460 (MXH98_06460)	1223110..1223280	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
MXH98_RS06465 (MXH98_06465)	1223281..1224027	-	747	WP_012117368.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
MXH98_RS06470 (MXH98_06470)	1224132..1225130	-	999	WP_007407255.1	inorganic phosphate transporter	-
MXH98_RS06475 (MXH98_06475)	1225143..1225760	-	618	WP_003154804.1	DUF47 domain-containing protein	-
MXH98_RS06480 (MXH98_06480)	1226042..1227358	-	1317	WP_007610842.1	amino acid permease	-
MXH98_RS06485 (MXH98_06485)	1227681..1228631	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29119.61 Da
• Isoelectric Point: 4.7003

>T242464 WP_012117368.1 NZ_CP095760:c1224027-1223281 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAEWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQADKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T242464 NZ_OW443151:368496-368759 [Vibrio cholerae]
ATGAGTAGTCGTTTACTGTCATGGACAGATGAGTCCTGGGATGACTACTTGTACTGGCAAACACAAGACAAAAAAACACT
CAAGCGGATCAATAAAATCATCAATGATGTTAAGCGTTCACCGTTTGAAGGCATTGGTAAACCAGAGCCTCTAAAAGAAA
ATCTATCTGGTTTTTGGTCTCGACGGATCGATGATACAAATAGACTGGTTTATGCTGTTGATGATAATGCAATAACGATT
ATTTCGTGTCGCTACCATTATTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT242464 WP_003154807.1 NZ_CP095760:c1223280-1223110 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT242464 NZ_OW443151:368249-368503 [Vibrio cholerae]
ATGAGAATCGTTTCTTTTACAGAAGCTAGGAATAGTTTAAAAGCAGTCTTGGATGCCGTTGTGAATGATGCGGATACAAC
CGTCATCACTCGACGTGATTCAGAAGATGCCGTGGTTATGTCGCTGGATTATTACAATAGTCTAATGGAAACCGTACATT
TGCTTCGCTCTCCAGCGAACGCTGAACATCTAAACCGTTCCATTGCCCAGTTCAAAGCGGGAAAAGTTACCCAAAGAGAT
CTGATTGATGAGTAG

Structures

Antitoxin

• AlphaFold DB: I2HQ14