Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 529541..530177 Replicon chromosome
Accession NZ_CP095759
Organism Bacillus safensis strain ZK-1

Toxin (Protein)


Gene name mazF Uniprot ID A0A0P7G713
Locus tag MXH81_RS02525 Protein ID WP_024425388.1
Coordinates 529827..530177 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag MXH81_RS02520 Protein ID WP_003214273.1
Coordinates 529541..529822 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MXH81_RS02500 (MXH81_02500) 525687..526292 - 606 WP_103132679.1 rhomboid family intramembrane serine protease -
MXH81_RS02505 (MXH81_02505) 526387..526752 + 366 WP_041089202.1 holo-ACP synthase -
MXH81_RS02510 (MXH81_02510) 526913..527929 + 1017 WP_087975235.1 outer membrane lipoprotein-sorting protein -
MXH81_RS02515 (MXH81_02515) 528071..529246 + 1176 WP_103132680.1 alanine racemase -
MXH81_RS02520 (MXH81_02520) 529541..529822 + 282 WP_003214273.1 hypothetical protein Antitoxin
MXH81_RS02525 (MXH81_02525) 529827..530177 + 351 WP_024425388.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
MXH81_RS02530 (MXH81_02530) 530294..531124 + 831 WP_044334717.1 RsbT co-antagonist protein RsbRA -
MXH81_RS02535 (MXH81_02535) 531129..531497 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
MXH81_RS02540 (MXH81_02540) 531500..531901 + 402 WP_073203956.1 anti-sigma regulatory factor -
MXH81_RS02545 (MXH81_02545) 531912..532919 + 1008 WP_024425391.1 PP2C family protein-serine/threonine phosphatase -
MXH81_RS02550 (MXH81_02550) 532979..533308 + 330 WP_024425392.1 anti-sigma factor antagonist -
MXH81_RS02555 (MXH81_02555) 533305..533793 + 489 WP_024425393.1 anti-sigma B factor RsbW -
MXH81_RS02560 (MXH81_02560) 533759..534547 + 789 WP_024425394.1 RNA polymerase sigma factor SigB -
MXH81_RS02565 (MXH81_02565) 534547..535146 + 600 WP_247721756.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8891

>T242462 WP_024425388.1 NZ_CP095759:529827-530177 [Bacillus safensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T242462 NZ_OW443151:c357159-356662 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATTGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGCGTAGCGTTCCCAGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT242462 WP_003214273.1 NZ_CP095759:529541-529822 [Bacillus safensis]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT242462 NZ_OW443151:c357428-357156 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCGGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0P7G713

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References