Detailed information of TA system TA242438

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1339893..1340809
• Replicon: chromosome
• Accession: NZ_CP095736
• Organism: Bacillus subtilis strain N4

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: O34853
• Locus tag: MX663_RS07025
• Protein ID: WP_003244695.1
• Coordinates: 1340063..1340809 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: -
• Locus tag: MX663_RS07020
• Protein ID: WP_088325699.1
• Coordinates: 1339893..1340063 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MX663_RS06985 (1336759)	1336759..1337085	+	327	WP_173614041.1	XkdW family protein	-
MX663_RS06990 (1337082)	1337082..1337246	+	165	WP_134155238.1	XkdX family protein	-
MX663_RS06995 (1337290)	1337290..1338129	+	840	WP_173614042.1	phage-like element PBSX protein XepA	-
MX663_RS07000 (1338182)	1338182..1338451	+	270	WP_003232655.1	hemolysin XhlA family protein	-
MX663_RS07005 (1338464)	1338464..1338727	+	264	WP_173614043.1	phage holin	-
MX663_RS07010 (1338740)	1338740..1339633	+	894	WP_247471567.1	N-acetylmuramoyl-L-alanine amidase	-
MX663_RS07015 (1339670)	1339670..1339807	-	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
MX663_RS07020 (1339893)	1339893..1340063	-	171	WP_088325699.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
MX663_RS07025 (1340063)	1340063..1340809	-	747	WP_003244695.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
MX663_RS07030 (1340919)	1340919..1341920	-	1002	WP_003232642.1	inorganic phosphate transporter	-
MX663_RS07035 (1341933)	1341933..1342550	-	618	WP_003218470.1	DUF47 domain-containing protein	-
MX663_RS07040 (1342826)	1342826..1344142	-	1317	WP_014479570.1	serine/threonine exchanger	-
MX663_RS07045 (1344531)	1344531..1345481	+	951	WP_014476561.1	ring-cleaving dioxygenase	-
MX663_RS07050 (1345590)	1345590..1345685	+	96	Protein_1326	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1305518..1406049	100531

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-55)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.50 Da
• Isoelectric Point: 4.6191

>T242438 WP_003244695.1 NZ_CP095736:c1340809-1340063 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T242438 NZ_OW443148:c420232-419942 [Vibrio cholerae]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6684.75 Da
• Isoelectric Point: 11.0203

>AT242438 WP_088325699.1 NZ_CP095736:c1340063-1339893 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKT

Nucleotide

• Length: 171 bp

>AT242438 NZ_OW443148:c420470-420222 [Vibrio cholerae]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGTGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCCAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Structures

Toxin

• PDB: 3O6Q