Detailed information of TA system TA242437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 532095..532731
• Replicon: chromosome
• Accession: NZ_CP095736
• Organism: Bacillus subtilis strain N4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: MX663_RS02760
• Protein ID: WP_003156187.1
• Coordinates: 532381..532731 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: MX663_RS02755
• Protein ID: WP_003225183.1
• Coordinates: 532095..532376 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MX663_RS02735 (528454)	528454..529053	-	600	WP_123372836.1	rhomboid family intramembrane serine protease	-
MX663_RS02740 (529148)	529148..529513	+	366	WP_014475874.1	holo-ACP synthase	-
MX663_RS02745 (529679)	529679..530695	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
MX663_RS02750 (530810)	530810..531979	+	1170	WP_106074084.1	alanine racemase	-
MX663_RS02755 (532095)	532095..532376	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
MX663_RS02760 (532381)	532381..532731	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
MX663_RS02765 (532846)	532846..533670	+	825	WP_015252765.1	RsbT co-antagonist protein RsbRA	-
MX663_RS02770 (533675)	533675..534040	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
MX663_RS02775 (534044)	534044..534445	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
MX663_RS02780 (534457)	534457..535464	+	1008	WP_015252763.1	phosphoserine phosphatase RsbU	-
MX663_RS02785 (535526)	535526..535855	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
MX663_RS02790 (535852)	535852..536334	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
MX663_RS02795 (536300)	536300..537088	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
MX663_RS02800 (537088)	537088..537687	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T242437 WP_003156187.1 NZ_CP095736:532381-532731 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T242437 NZ_OW443148:414600-414887 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT242437 WP_003225183.1 NZ_CP095736:532095-532376 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT242437 NZ_OW443148:414898-415215 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32