Detailed information of TA system TA242436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 748708..750271
• Replicon: chromosome
• Accession: NZ_CP095733
• Organism: Methylophaga pinxianii strain TMB1265

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LGT42_RS03620
• Protein ID: WP_226071568.1
• Coordinates: 748708..750018 (-)
• Length: 437 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LGT42_RS03625
• Protein ID: WP_014705941.1
• Coordinates: 750020..750271 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGT42_RS03590 (LGT42_003590)	744056..745306	+	1251	WP_247363602.1	sigma-54 dependent transcriptional regulator	-
LGT42_RS03595 (LGT42_003595)	745810..746163	+	354	WP_226071556.1	hypothetical protein	-
LGT42_RS03600 (LGT42_003600)	746305..746598	+	294	WP_226071558.1	hypothetical protein	-
LGT42_RS03605 (LGT42_003605)	746598..746765	+	168	WP_226071559.1	hypothetical protein	-
LGT42_RS03610 (LGT42_003610)	747256..747879	+	624	WP_226071564.1	hypothetical protein	-
LGT42_RS03615 (LGT42_003615)	747985..748188	+	204	WP_226071566.1	hypothetical protein	-
LGT42_RS03620 (LGT42_003620)	748708..750018	-	1311	WP_226071568.1	type II toxin-antitoxin system HipA family toxin	Toxin
LGT42_RS03625 (LGT42_003625)	750020..750271	-	252	WP_014705941.1	helix-turn-helix domain-containing protein	Antitoxin
LGT42_RS03630 (LGT42_003630)	750533..751228	+	696	WP_226071569.1	hypothetical protein	-
LGT42_RS03635 (LGT42_003635)	751285..752037	-	753	WP_226071572.1	IS21-like element helper ATPase IstB	-
LGT42_RS03640 (LGT42_003640)	752030..753523	-	1494	WP_226071574.1	IS21 family transposase	-
LGT42_RS03645 (LGT42_003645)	753738..754238	+	501	WP_226071580.1	hypothetical protein	-
LGT42_RS03650 (LGT42_003650)	754269..754463	-	195	WP_226071585.1	MbcA/ParS/Xre antitoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-66)

Sequences

Toxin

Protein

• Length: 437 a.a.
• Molecular weight: 49163.27 Da
• Isoelectric Point: 6.6380

>T242436 WP_226071568.1 NZ_CP095733:c750018-748708 [Methylophaga pinxianii]
MGRRQHYIPLDVWISNRIVGQLIRESTGAISFIYDQRWLDWEHAFPVSLSLPLRKDRFIGARVIPVFENLLPDNDGVRKR
LAERVGAEGIDAFSLLSVIGRDCIGALQFLPQGSSQETSNQLEGTVLTEHEIEVLLGELDEVPLGLQKENDFRISVAGAQ
EKTALLFHNGEWIKPTGTTPTTHILKPQIGKLSNGMDLTHSVENEFLCLKLLKNFGLRTANTEIIDFGSRRALVIERFDR
RWTADGRLIRLPQEDCCQALSIPPTRKYQSEGGPGIVEIMDLFRGSDEPTQDRMDFFMSTILFWAIGATDGHGKNFSLSL
MPGGRYRLTPLYDVLTLQPSVDAKQVAHKNYKLSMSFGKSRHYRIKEIAVRHIIETGVEAGLSRESIAEIFDQLCKDKDK
AIEHTLKGLPKDFPQNLLDSTFTALEKHIALLDNSR

Nucleotide

• Length: 1311 bp

>T242436 NZ_OW443148:c410842-410525 [Vibrio cholerae]
ATGCAAAATAAACAATATAAGCTCAGCCAATTAGCCCAAGAGCACTTACTCAAGATTAAACACTACACCATTGAAAATTT
CGCTGAAGCGCAGTGGCAAAAATATAAGTCGACCTTGCTTTCAGGTTTCCAAACTCTTGCGGATAACCCAGGACTAGGAA
AAAGCTGTGAAGATATTTACCAAAATGGTTTTTACTTTCCAGTGGGGAAACACATGGCTTATTACACCAAAGAAGCAAAC
TTCATTCTCATTGTTGCGGTATTAGGGCAATCACAACTGCCCCAAAAGCATCTCAAACAGTCACGCTTTGTTTCTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9231.62 Da
• Isoelectric Point: 8.7278

>AT242436 WP_014705941.1 NZ_CP095733:c750271-750020 [Methylophaga pinxianii]
MKILVRSPKDLGNAIRHARKAKALTQSELASKSGIWQETISKIENGVSSTKLDTVFDLFAALDLEIHIQERSKGNISQLE
EIL

Nucleotide

• Length: 252 bp

>AT242436 NZ_OW443148:c411103-410861 [Vibrio cholerae]
ATGCACACACTAACAGCAAATGATGCTAAACGAAATTTTGGTGAACTTCTTCTAAGCGCACAACGCGAACCTGTCATCAT
CAGCAAAAACAGTAAGAATACTGTCGTTGTCATGTCCATTAAGGACTTTGAAGAACTTGAAGCAATGAAACTCGATTATC
TCAAACACTGCTTTGAGTCTGCTCAGAAAGACTTAGATAGTGGCAAAACCGTTGATGGTGCCACTTTTCTAAACACCCTT
TGA