Detailed information of TA system TA242431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 193401..194030
• Replicon: chromosome
• Accession: NZ_CP095726
• Organism: Listeria innocua strain 2010L-2059

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: Q92DC7
• Locus tag: QMF10_RS03305
• Protein ID: WP_010990666.1
• Coordinates: 193683..194030 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A7U7S1X0
• Locus tag: QMF10_RS03300
• Protein ID: WP_003766128.1
• Coordinates: 193401..193679 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMF10_RS03280 (EWI69_03285)	188698..190182	+	1485	WP_207577334.1	PH domain-containing protein	-
QMF10_RS03285 (EWI69_03290)	190333..191712	+	1380	WP_003766122.1	protoporphyrinogen oxidase	-
QMF10_RS03290 (EWI69_03295)	191714..192070	+	357	WP_003766124.1	holo-ACP synthase	-
QMF10_RS03295 (EWI69_03300)	192089..193195	+	1107	WP_003766126.1	alanine racemase	-
QMF10_RS03300 (EWI69_03305)	193401..193679	+	279	WP_003766128.1	CopG family ribbon-helix-helix protein	Antitoxin
QMF10_RS03305 (EWI69_03310)	193683..194030	+	348	WP_010990666.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
QMF10_RS03310 (EWI69_03315)	194280..195116	+	837	WP_003766130.1	RsbT co-antagonist protein RsbRA	-
QMF10_RS03315 (EWI69_03320)	195122..195478	+	357	WP_003721457.1	STAS domain-containing protein	-
QMF10_RS03320 (EWI69_03325)	195481..195891	+	411	WP_207577335.1	anti-sigma regulatory factor	-
QMF10_RS03325 (EWI69_03330)	195908..196912	+	1005	WP_003761312.1	PP2C family protein-serine/threonine phosphatase	-
QMF10_RS03330 (EWI69_03335)	197062..197406	+	345	WP_003721460.1	anti sigma b factor antagonist RsbV	-
QMF10_RS03335 (EWI69_03340)	197390..197863	+	474	WP_003761314.1	anti-sigma B factor RsbW	-
QMF10_RS03340 (EWI69_03345)	197841..198620	+	780	WP_207577336.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12801.85 Da
• Isoelectric Point: 6.4735

>T242431 WP_010990666.1 NZ_CP095726:193683-194030 [Listeria innocua]
MMVKRGDVYYADLSPVVGSEQGGIRPVLIIQNDIGNRFSPTVIVAAITAKIQKAKLPTHVEATRKDGFERDSVILLEQIR
TIDKQRLTDKITHLDEELMAKVNQALEVSLGVVEF

Nucleotide

• Length: 348 bp

>T242431 NZ_OW443148:c370246-369749 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10575.05 Da
• Isoelectric Point: 4.2980

>AT242431 WP_003766128.1 NZ_CP095726:193401-193679 [Listeria innocua]
VLEKEKRTIISVELTQEMVQELDVVVEKEKMGRSEVIMEATQQFLQEKRARELRDEMERGYAEMATINFAIACECTHVEA
EAEDRNISILGG

Nucleotide

• Length: 279 bp

>AT242431 NZ_OW443148:c370515-370243 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Structures

Toxin

• AlphaFold DB: A0A1E7E4N0

Antitoxin

• AlphaFold DB: A0A7U7S1X0