Detailed information of TA system TA242427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 55117..55642
• Replicon: plasmid pIncFIA_IncFII
• Accession: NZ_CP095559
• Organism: Klebsiella pneumoniae strain BA11088

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: MXX37_RS26980
• Protein ID: WP_001159868.1
• Coordinates: 55337..55642 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: MXX37_RS26975
• Protein ID: WP_000813634.1
• Coordinates: 55117..55335 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MXX37_RS26945 (MXX37_26900)	50510..50926	-	417	WP_001034046.1	type II toxin-antitoxin system VapC family toxin	-
MXX37_RS26950 (MXX37_26905)	50923..51153	-	231	WP_001261278.1	type II toxin-antitoxin system VapB family antitoxin	-
MXX37_RS26955 (MXX37_26910)	51418..51918	+	501	WP_000528931.1	HEPN family nuclease	-
MXX37_RS26960 (MXX37_26915)	51931..52704	+	774	WP_000905949.1	hypothetical protein	-
MXX37_RS26965 (MXX37_26920)	52871..54004	+	1134	WP_000545987.1	DUF3800 domain-containing protein	-
MXX37_RS26970 (MXX37_26925)	54038..54526	-	489	WP_011254646.1	hypothetical protein	-
MXX37_RS26975 (MXX37_26930)	55117..55335	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
MXX37_RS26980 (MXX37_26935)	55337..55642	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
MXX37_RS26985 (MXX37_26940)	55643..56449	+	807	WP_000016982.1	site-specific integrase	-
MXX37_RS26990 (MXX37_26945)	57223..57978	+	756	WP_000852146.1	replication initiation protein RepE	-
MXX37_RS26995 (MXX37_26950)	58566..59732	+	1167	WP_000772446.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / mph(A) / sul1 / qacE / aadA2 / dfrA12 / sitABCD	iucA / iucB / iucC / iucD / iutA	1..73277	73277

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T242427 WP_001159868.1 NZ_CP095559:55337-55642 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T242427 NZ_OW370493:c566366-566085 [Rickettsia endosymbiont of Oedothorax gibbosus]
ATGCGAACGATTAAGCGTACTACACAATTTAAACGTGATTATACAAGAGAAAAACGTAGACAACATGGCGTAACTTTGGA
TAATGATTTATCGAAAGCGATTGAATTGTTAGTCATTGATGCACAATTACCTAAATGCATGCGTGAGCATCCTCTCATCG
GTGATTGGAAAGATTGCCGAGATTGTCATATTAAATCCGATCTTGTGCTTATCTATCAGAAGTCGGATGATTACACGCTG
AAATTAATTCGCCTTGGTTCACACAGTGAACTTAGTTTATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT242427 WP_000813634.1 NZ_CP095559:55117-55335 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT242427 NZ_OW370493:c566616-566353 [Rickettsia endosymbiont of Oedothorax gibbosus]
ATGGCTGTTAATACTACTGTTCGTGCTCGCATTGATGAGAATATAAAAGAGGAAGCTTCTATTGTACTATCAACTATGGG
GCTTACTGTATCAGATGCTTTTCGTATGCTTCTAACCCGTATTGCACGCGAAAAAGCCTTGCCTTTTGATCCACTTGTAC
CAAATGCTAAAACAATTGAGGCTATAAAAGCCGCACGAAGTGGTAAACTAATAACTATTGGTAGCATAAATAATTTATTA
ACTGATTTAAATGCGAACGATTAA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2