Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5261946..5262571 Replicon chromosome
Accession NZ_CP095557
Organism Klebsiella pneumoniae strain BA11088

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MXX37_RS25635 Protein ID WP_002882817.1
Coordinates 5261946..5262329 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MXX37_RS25640 Protein ID WP_004150355.1
Coordinates 5262329..5262571 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MXX37_RS25620 (5259312) 5259312..5260214 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MXX37_RS25625 (5260211) 5260211..5260846 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MXX37_RS25630 (5260843) 5260843..5261772 + 930 WP_064162495.1 formate dehydrogenase accessory protein FdhE -
MXX37_RS25635 (5261946) 5261946..5262329 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MXX37_RS25640 (5262329) 5262329..5262571 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MXX37_RS25645 (5262776) 5262776..5263693 + 918 WP_004181612.1 alpha/beta hydrolase -
MXX37_RS25650 (5263707) 5263707..5264648 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MXX37_RS25655 (5264693) 5264693..5265130 - 438 WP_064162494.1 D-aminoacyl-tRNA deacylase -
MXX37_RS25660 (5265127) 5265127..5265987 - 861 WP_004146232.1 virulence factor BrkB family protein -
MXX37_RS25665 (5265981) 5265981..5266580 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T242424 WP_002882817.1 NZ_CP095557:c5262329-5261946 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T242424 NZ_OW052571:c3994696-3994394 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT242424 WP_004150355.1 NZ_CP095557:c5262571-5262329 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT242424 NZ_OW052571:c3994920-3994693 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References