Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1971187..1971841 Replicon chromosome
Accession NZ_CP095540
Organism Escherichia coli strain GN03204

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag MW399_RS09475 Protein ID WP_000244765.1
Coordinates 1971434..1971841 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID F4T2L5
Locus tag MW399_RS09470 Protein ID WP_000354050.1
Coordinates 1971187..1971453 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW399_RS09450 (1967275) 1967275..1968708 - 1434 WP_001296350.1 6-phospho-beta-glucosidase BglA -
MW399_RS09455 (1968753) 1968753..1969064 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
MW399_RS09460 (1969228) 1969228..1969887 + 660 WP_000250275.1 hemolysin III family protein -
MW399_RS09465 (1969964) 1969964..1970944 - 981 WP_000886080.1 tRNA-modifying protein YgfZ -
MW399_RS09470 (1971187) 1971187..1971453 + 267 WP_000354050.1 FAD assembly factor SdhE Antitoxin
MW399_RS09475 (1971434) 1971434..1971841 + 408 WP_000244765.1 protein YgfX Toxin
MW399_RS09480 (1971881) 1971881..1972402 - 522 WP_001055874.1 flavodoxin FldB -
MW399_RS09485 (1972514) 1972514..1973410 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MW399_RS09490 (1973435) 1973435..1974145 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MW399_RS09495 (1974151) 1974151..1975884 + 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T242389 WP_000244765.1 NZ_CP095540:1971434-1971841 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T242389 NZ_OW052571:c2106987-2106589 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10535.07 Da        Isoelectric Point: 5.1599

>AT242389 WP_000354050.1 NZ_CP095540:1971187-1971453 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT242389 NZ_OW052571:c2107215-2106988 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
AlphaFold DB A0A061L3F4

References