Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 458307..458804 Replicon chromosome
Accession NZ_CP095540
Organism Escherichia coli strain GN03204

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MW399_RS02255 Protein ID WP_001173343.1
Coordinates 458307..458480 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag MW399_RS02260 Protein ID WP_001312331.1
Coordinates 458508..458804 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW399_RS02245 (454977) 454977..456434 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
MW399_RS02250 (456671) 456671..458188 + 1518 WP_000003806.1 lysine--tRNA ligase -
MW399_RS02255 (458307) 458307..458480 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MW399_RS02260 (458508) 458508..458804 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MW399_RS02265 (459032) 459032..459304 - 273 WP_000405643.1 GNAT family N-acetyltransferase -
MW399_RS02270 (459518) 459518..463447 - 3930 WP_001080127.1 autotransporter outer membrane beta-barrel domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 458307..474487 16180


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T242383 WP_001173343.1 NZ_CP095540:c458480-458307 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T242383 NZ_OW052571:c1497060-1496647 [Mycobacterium tuberculosis]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT242383 WP_001312331.1 NZ_CP095540:c458804-458508 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT242383 NZ_OW052571:c1496608-1496351 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References