Detailed information of TA system TA242382

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 249936..250770
• Replicon: chromosome
• Accession: NZ_CP095540
• Organism: Escherichia coli strain GN03204

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1J6XFW9
• Locus tag: MW399_RS01220
• Protein ID: WP_000854770.1
• Coordinates: 249936..250313 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M1N885
• Locus tag: MW399_RS01225
• Protein ID: WP_001280950.1
• Coordinates: 250402..250770 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW399_RS01195 (246047)	246047..247669	-	1623	WP_001295726.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-
MW399_RS01200 (248460)	248460..248636	-	177	Protein_236	helix-turn-helix domain-containing protein	-
MW399_RS01205 (249003)	249003..249152	-	150	Protein_237	hypothetical protein	-
MW399_RS01210 (249258)	249258..249434	-	177	WP_000839286.1	DUF957 domain-containing protein	-
MW399_RS01215 (249451)	249451..249939	-	489	WP_000761690.1	DUF5983 family protein	-
MW399_RS01220 (249936)	249936..250313	-	378	WP_000854770.1	TA system toxin CbtA family protein	Toxin
MW399_RS01225 (250402)	250402..250770	-	369	WP_001280950.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MW399_RS01230 (250933)	250933..251154	-	222	WP_001220314.1	DUF987 domain-containing protein	-
MW399_RS01235 (251217)	251217..251693	-	477	WP_001186779.1	RadC family protein	-
MW399_RS01240 (251709)	251709..252173	-	465	WP_000855061.1	antirestriction protein	-
MW399_RS01245 (252515)	252515..253333	-	819	WP_001234712.1	DUF932 domain-containing protein	-
MW399_RS01250 (253451)	253451..253646	-	196	Protein_246	DUF905 family protein	-
MW399_RS01255 (253717)	253717..255618	-	1902	Protein_247	Ag43/Cah family autotransporter adhesin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	238290..278499	40209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.37 Da
• Isoelectric Point: 8.7438

>T242382 WP_000854770.1 NZ_CP095540:c250313-249936 [Escherichia coli]
MKTLPDTHVRKVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T242382 NZ_OW052571:c1496354-1495977 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13737.56 Da
• Isoelectric Point: 7.0268

>AT242382 WP_001280950.1 NZ_CP095540:c250770-250402 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAFYPTSETKK

Nucleotide

• Length: 369 bp

>AT242382 NZ_OW052571:c1496608-1496351 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A1J6XFW9

Antitoxin

• AlphaFold DB: A0A1M1N885