Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 179080..179656 Replicon chromosome
Accession NZ_CP095540
Organism Escherichia coli strain GN03204

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag MW399_RS00855 Protein ID WP_001297643.1
Coordinates 179369..179656 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag MW399_RS00850 Protein ID WP_000063156.1
Coordinates 179080..179382 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW399_RS00835 (175676) 175676..177826 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
MW399_RS00840 (177876) 177876..178079 + 204 WP_000467859.1 YbdD/YjiX family protein -
MW399_RS00845 (178090) 178090..179046 + 957 WP_001297640.1 GTPase -
MW399_RS00850 (179080) 179080..179382 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
MW399_RS00855 (179369) 179369..179656 - 288 WP_001297643.1 BrnT family toxin Toxin
MW399_RS00860 (179855) 179855..180552 - 698 Protein_168 IS1 family transposase -
MW399_RS00865 (180609) 180609..180704 + 96 Protein_169 dienelactone hydrolase family protein -
MW399_RS00870 (180850) 180850..182082 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
MW399_RS00875 (182123) 182123..183403 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 179855..180064 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T242381 WP_001297643.1 NZ_CP095540:c179656-179369 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T242381 NZ_OW052571:1495092-1495487 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT242381 WP_000063156.1 NZ_CP095540:c179382-179080 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT242381 NZ_OW052571:1494850-1495095 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References