242379

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 14984..15509 Replicon plasmid p4592
Accession NZ_CP095539
Organism Escherichia coli strain GN04592

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag MW396_RS25625 Protein ID WP_001159871.1
Coordinates 14984..15289 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag MW396_RS25630 Protein ID WP_000813634.1
Coordinates 15291..15509 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW396_RS25590 (10411) 10411..11208 + 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -
MW396_RS25595 (11270) 11270..11521 + 252 Protein_16 DUF4113 domain-containing protein -
MW396_RS25600 (11526) 11526..11918 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
MW396_RS25605 (11923) 11923..12894 - 972 WP_001103694.1 plasmid segregation protein ParM -
MW396_RS25610 (13123) 13123..13767 + 645 WP_000633911.1 AAA family ATPase -
MW396_RS25615 (13761) 13761..14036 + 276 WP_000239529.1 hypothetical protein -
MW396_RS25620 (14174) 14174..14983 - 810 WP_000016979.1 site-specific integrase -
MW396_RS25625 (14984) 14984..15289 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
MW396_RS25630 (15291) 15291..15509 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MW396_RS25635 (17831) 17831..18277 + 447 WP_251988392.1 hypothetical protein -
MW396_RS25640 (18341) 18341..19687 + 1347 WP_000483767.1 IS4-like element IS4 family transposase -
MW396_RS25645 (19778) 19778..20098 - 321 WP_001363097.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B senB 1..135067 135067
- flank IS/Tn - - 18359..19687 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T242379 WP_001159871.1 NZ_CP095539:c15289-14984 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T242379 NZ_OW052571:1389663-1389986 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT242379 WP_000813634.1 NZ_CP095539:c15509-15291 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT242379 NZ_OW052571:1389338-1389583 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References