Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 14984..15509 Replicon plasmid p4592
Accession NZ_CP095539
Organism Escherichia coli strain GN04592

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag MW396_RS25625 Protein ID WP_001159871.1
Coordinates 14984..15289 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag MW396_RS25630 Protein ID WP_000813634.1
Coordinates 15291..15509 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW396_RS25590 (10411) 10411..11208 + 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -
MW396_RS25595 (11270) 11270..11521 + 252 Protein_16 DUF4113 domain-containing protein -
MW396_RS25600 (11526) 11526..11918 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
MW396_RS25605 (11923) 11923..12894 - 972 WP_001103694.1 plasmid segregation protein ParM -
MW396_RS25610 (13123) 13123..13767 + 645 WP_000633911.1 AAA family ATPase -
MW396_RS25615 (13761) 13761..14036 + 276 WP_000239529.1 hypothetical protein -
MW396_RS25620 (14174) 14174..14983 - 810 WP_000016979.1 site-specific integrase -
MW396_RS25625 (14984) 14984..15289 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
MW396_RS25630 (15291) 15291..15509 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MW396_RS25635 (17831) 17831..18277 + 447 WP_251988392.1 hypothetical protein -
MW396_RS25640 (18341) 18341..19687 + 1347 WP_000483767.1 IS4-like element IS4 family transposase -
MW396_RS25645 (19778) 19778..20098 - 321 WP_001363097.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B senB 1..135067 135067
- flank IS/Tn - - 18359..19687 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T242379 WP_001159871.1 NZ_CP095539:c15289-14984 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T242379 NZ_OW052571:1389663-1389986 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT242379 WP_000813634.1 NZ_CP095539:c15509-15291 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT242379 NZ_OW052571:1389338-1389583 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References