Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 5021057..5021669 Replicon chromosome
Accession NZ_CP095538
Organism Escherichia coli strain GN04592

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag MW396_RS24505 Protein ID WP_000833473.1
Coordinates 5021057..5021242 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID L4IWR9
Locus tag MW396_RS24510 Protein ID WP_000499744.1
Coordinates 5021259..5021669 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW396_RS24495 (5017889) 5017889..5019040 + 1152 WP_000741501.1 L-threonine dehydrogenase -
MW396_RS24500 (5019241) 5019241..5020779 + 1539 WP_001309845.1 aldehyde dehydrogenase AldB -
MW396_RS24505 (5021057) 5021057..5021242 + 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
MW396_RS24510 (5021259) 5021259..5021669 + 411 WP_000499744.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MW396_RS24515 (5021741) 5021741..5023705 - 1965 WP_001026861.1 glycoside hydrolase family 127 protein -
MW396_RS24520 (5023716) 5023716..5025116 - 1401 WP_000204802.1 MFS transporter -
MW396_RS24525 (5025342) 5025342..5026157 + 816 WP_000891825.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T242378 WP_000833473.1 NZ_CP095538:5021057-5021242 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T242378 NZ_OW052571:1295828-1296244 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15241.15 Da        Isoelectric Point: 4.5486

>AT242378 WP_000499744.1 NZ_CP095538:5021259-5021669 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT242378 NZ_OW052571:1295565-1295831 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB L4IWR9

References