Detailed information of TA system TA242366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2782231..2783015
• Replicon: chromosome
• Accession: NZ_CP095538
• Organism: Escherichia coli strain GN04592

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: MW396_RS13910
• Protein ID: WP_000613626.1
• Coordinates: 2782521..2783015 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: MW396_RS13905
• Protein ID: WP_001110447.1
• Coordinates: 2782231..2782524 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW396_RS13895 (2777381)	2777381..2778340	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
MW396_RS13900 (2778913)	2778913..2782098	+	3186	WP_000827427.1	ribonuclease E	-
MW396_RS13905 (2782231)	2782231..2782524	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
MW396_RS13910 (2782521)	2782521..2783015	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
MW396_RS13915 (2783110)	2783110..2784063	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
MW396_RS13920 (2784075)	2784075..2785718	-	1644	WP_000096524.1	flagellar hook-associated protein FlgK	-
MW396_RS13925 (2785784)	2785784..2786725	-	942	WP_001309406.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
MW396_RS13930 (2786725)	2786725..2787822	-	1098	WP_000589320.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T242366 WP_000613626.1 NZ_CP095538:2782521-2783015 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T242366 NZ_OW052570:c4406792-4406394 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT242366 WP_001110447.1 NZ_CP095538:2782231-2782524 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT242366 NZ_OW052570:c4407020-4406793 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6