Detailed information of TA system TA242365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2282298..2282936
• Replicon: chromosome
• Accession: NZ_CP095538
• Organism: Escherichia coli strain GN04592

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: MW396_RS10965
• Protein ID: WP_000813797.1
• Coordinates: 2282760..2282936 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: MW396_RS10960
• Protein ID: WP_076611057.1
• Coordinates: 2282298..2282714 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW396_RS10940 (2277450)	2277450..2278391	-	942	WP_001251335.1	ABC transporter permease	-
MW396_RS10945 (2278392)	2278392..2279405	-	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
MW396_RS10950 (2279423)	2279423..2280568	-	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
MW396_RS10955 (2280813)	2280813..2282219	-	1407	WP_064772446.1	PLP-dependent aminotransferase family protein	-
MW396_RS10960 (2282298)	2282298..2282714	-	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
MW396_RS10965 (2282760)	2282760..2282936	-	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
MW396_RS10970 (2283158)	2283158..2283388	+	231	WP_000494244.1	YncJ family protein	-
MW396_RS10975 (2283480)	2283480..2285441	-	1962	WP_024189226.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
MW396_RS10980 (2285514)	2285514..2286050	-	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
MW396_RS10985 (2286142)	2286142..2287314	+	1173	WP_001236223.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T242365 WP_000813797.1 NZ_CP095538:c2282936-2282760 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T242365 NZ_OW052570:c4339010-4338600 [Mycobacterium tuberculosis]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCCCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT242365 WP_076611057.1 NZ_CP095538:c2282714-2282298 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT242365 NZ_OW052570:c4339236-4339003 [Mycobacterium tuberculosis]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A829J715