Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 642424..643078 Replicon chromosome
Accession NZ_CP095538
Organism Escherichia coli strain GN04592

Toxin (Protein)


Gene name cptA Uniprot ID B7N7E0
Locus tag MW396_RS03235 Protein ID WP_000244769.1
Coordinates 642671..643078 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MW396_RS03230 Protein ID WP_000354046.1
Coordinates 642424..642690 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW396_RS03210 (638512) 638512..639945 - 1434 WP_001513393.1 6-phospho-beta-glucosidase BglA -
MW396_RS03215 (639990) 639990..640301 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
MW396_RS03220 (640465) 640465..641124 + 660 WP_000250274.1 hemolysin III family protein -
MW396_RS03225 (641201) 641201..642181 - 981 WP_000886049.1 tRNA-modifying protein YgfZ -
MW396_RS03230 (642424) 642424..642690 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MW396_RS03235 (642671) 642671..643078 + 408 WP_000244769.1 protein YgfX Toxin
MW396_RS03240 (643118) 643118..643639 - 522 WP_001055874.1 flavodoxin FldB -
MW396_RS03245 (643751) 643751..644647 + 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
MW396_RS03250 (644672) 644672..645382 + 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MW396_RS03255 (645388) 645388..647121 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16062.03 Da        Isoelectric Point: 11.5554

>T242352 WP_000244769.1 NZ_CP095538:642671-643078 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGK
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T242352 NZ_OW052570:3533176-3533568 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT242352 WP_000354046.1 NZ_CP095538:642424-642690 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT242352 NZ_OW052570:3532964-3533179 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J3T1

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References