Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4355824..4356478 Replicon chromosome
Accession NZ_CP095533
Organism Escherichia coli strain GN02461

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag MW400_RS20390 Protein ID WP_000244781.1
Coordinates 4355824..4356231 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MW400_RS20395 Protein ID WP_000354046.1
Coordinates 4356212..4356478 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW400_RS20370 (4351781) 4351781..4353514 - 1734 WP_000813226.1 single-stranded-DNA-specific exonuclease RecJ -
MW400_RS20375 (4353520) 4353520..4354230 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MW400_RS20380 (4354255) 4354255..4355151 - 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
MW400_RS20385 (4355263) 4355263..4355784 + 522 WP_001055874.1 flavodoxin FldB -
MW400_RS20390 (4355824) 4355824..4356231 - 408 WP_000244781.1 protein YgfX Toxin
MW400_RS20395 (4356212) 4356212..4356478 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MW400_RS20400 (4356721) 4356721..4357701 + 981 WP_000886086.1 tRNA-modifying protein YgfZ -
MW400_RS20405 (4357778) 4357778..4358437 - 660 WP_000250269.1 hemolysin III family protein -
MW400_RS20410 (4358601) 4358601..4358912 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
MW400_RS20415 (4358957) 4358957..4360390 + 1434 WP_001559758.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T242343 WP_000244781.1 NZ_CP095533:c4356231-4355824 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T242343 NZ_OW052570:c1890026-1889724 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT242343 WP_000354046.1 NZ_CP095533:c4356478-4356212 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT242343 NZ_OW052570:c1890250-1890023 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References