Detailed information of TA system TA242332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2785028..2786616
• Replicon: chromosome
• Accession: NZ_CP095533
• Organism: Escherichia coli strain GN02461

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: V0Z3Q2
• Locus tag: MW400_RS12980
• Protein ID: WP_001125418.1
• Coordinates: 2785028..2786350 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: MW400_RS12985
• Protein ID: WP_001327997.1
• Coordinates: 2786350..2786616 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW400_RS12940 (2780062)	2780062..2780634	-	573	WP_000789912.1	NAD(P)H-dependent oxidoreductase	-
MW400_RS12945 (2780785)	2780785..2780937	-	153	WP_001322417.1	amidohydrolase family protein	-
MW400_RS12950 (2780927)	2780927..2781346	-	420	WP_001327999.1	amidohydrolase family protein	-
MW400_RS12955 (2781306)	2781306..2781461	-	156	WP_001327998.1	hypothetical protein	-
MW400_RS12960 (2781564)	2781564..2782511	-	948	WP_000854796.1	aldo/keto reductase	-
MW400_RS12965 (2782585)	2782585..2783274	-	690	WP_000801313.1	type 1 glutamine amidotransferase domain-containing protein	-
MW400_RS12970 (2783283)	2783283..2783591	-	309	WP_001221046.1	hypothetical protein	-
MW400_RS12975 (2783730)	2783730..2784629	+	900	WP_001057776.1	LysR family transcriptional regulator	-
MW400_RS12980 (2785028)	2785028..2786350	-	1323	WP_001125418.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
MW400_RS12985 (2786350)	2786350..2786616	-	267	WP_001327997.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49316.95 Da
• Isoelectric Point: 8.5823

>T242332 WP_001125418.1 NZ_CP095533:c2786350-2785028 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T242332 NZ_OW052570:c1534413-1534006 [Mycobacterium tuberculosis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9975.33 Da
• Isoelectric Point: 6.2156

>AT242332 WP_001327997.1 NZ_CP095533:c2786616-2786350 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSGLAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT242332 NZ_OW052570:c1534670-1534410 [Mycobacterium tuberculosis]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Structures

Toxin

• AlphaFold DB: V0Z3Q2