Detailed information of TA system TA242331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2683997..2684635
• Replicon: chromosome
• Accession: NZ_CP095533
• Organism: Escherichia coli strain GN02461

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: MW400_RS12540
• Protein ID: WP_000813797.1
• Coordinates: 2683997..2684173 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: MW400_RS12545
• Protein ID: WP_001270286.1
• Coordinates: 2684219..2684635 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW400_RS12520 (2679619)	2679619..2680830	-	1212	WP_001327922.1	BenE family transporter YdcO	-
MW400_RS12525 (2680883)	2680883..2681419	+	537	WP_000429505.1	DNA-binding transcriptional regulator SutR	-
MW400_RS12530 (2681492)	2681492..2683453	+	1962	WP_001445698.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
MW400_RS12535 (2683545)	2683545..2683775	-	231	WP_000491976.1	YncJ family protein	-
MW400_RS12540 (2683997)	2683997..2684173	+	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
MW400_RS12545 (2684219)	2684219..2684635	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
MW400_RS12550 (2684714)	2684714..2686120	+	1407	WP_000760596.1	PLP-dependent aminotransferase family protein	-
MW400_RS12555 (2686365)	2686365..2687510	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
MW400_RS12560 (2687528)	2687528..2688541	+	1014	WP_000220398.1	ABC transporter ATP-binding protein	-
MW400_RS12565 (2688542)	2688542..2689483	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T242331 WP_000813797.1 NZ_CP095533:2683997-2684173 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T242331 NZ_OW052570:1498795-1499178 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT242331 WP_001270286.1 NZ_CP095533:2684219-2684635 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT242331 NZ_OW052570:1498545-1498700 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Structures

Toxin

• AlphaFold DB: A0A829J715