Detailed information of TA system TA242326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1960933..1961638
• Replicon: chromosome
• Accession: NZ_CP095533
• Organism: Escherichia coli strain GN02461

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: MW400_RS08885
• Protein ID: WP_000539521.1
• Coordinates: 1961252..1961638 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MW400_RS08880
• Protein ID: WP_001280945.1
• Coordinates: 1960933..1961262 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW400_RS08865 (1956090)	1956090..1957001	+	912	WP_001236018.1	glutathione ABC transporter permease GsiD	-
MW400_RS08870 (1957179)	1957179..1959527	+	2349	WP_001328220.1	EAL domain-containing protein	-
MW400_RS08875 (1959535)	1959535..1960863	+	1329	WP_000086868.1	GGDEF domain-containing protein	-
MW400_RS08880 (1960933)	1960933..1961262	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
MW400_RS08885 (1961252)	1961252..1961638	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MW400_RS08890 (1961864)	1961864..1963189	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
MW400_RS08895 (1963402)	1963402..1963785	+	384	WP_000497137.1	biofilm formation regulator BssR	-
MW400_RS08900 (1963896)	1963896..1965011	+	1116	WP_000555049.1	aldose sugar dehydrogenase YliI	-
MW400_RS08905 (1965008)	1965008..1965634	-	627	WP_001328219.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T242326 WP_000539521.1 NZ_CP095533:c1961638-1961252 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T242326 NZ_OW052570:c1238120-1237686 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT242326 WP_001280945.1 NZ_CP095533:c1961262-1960933 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT242326 NZ_OW052570:c1238305-1238126 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCAGGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Toxin

• AlphaFold DB: A0A829GCT6