Detailed information of TA system TA242321

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 917113..917820
• Replicon: chromosome
• Accession: NZ_CP095533
• Organism: Escherichia coli strain GN02461

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: V0YJ99
• Locus tag: MW400_RS04150
• Protein ID: WP_000691792.1
• Coordinates: 917479..917820 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0YMY8
• Locus tag: MW400_RS04145
• Protein ID: WP_000939436.1
• Coordinates: 917113..917448 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW400_RS04120 (913060)	913060..913278	-	219	WP_000070772.1	AlpA family phage regulatory protein	-
MW400_RS04125 (913396)	913396..914010	-	615	WP_000772910.1	inovirus Gp2 family protein	-
MW400_RS04130 (914833)	914833..915555	+	723	WP_059331820.1	hypothetical protein	-
MW400_RS04135 (915792)	915792..916610	+	819	WP_001046747.1	DUF932 domain-containing protein	-
MW400_RS04140 (916640)	916640..917113	+	474	WP_001292743.1	DNA repair protein RadC	-
MW400_RS04145 (917113)	917113..917448	+	336	WP_000939436.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MW400_RS04150 (917479)	917479..917820	+	342	WP_000691792.1	TA system toxin CbtA family protein	Toxin
MW400_RS04155 (917932)	917932..918765	+	834	WP_001192747.1	DUF4942 domain-containing protein	-
MW400_RS04160 (918835)	918835..919230	+	396	WP_000152741.1	DUF6088 family protein	-
MW400_RS04165 (919223)	919223..920170	+	948	WP_000342498.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
MW400_RS04170 (920208)	920208..920393	-	186	WP_000066585.1	hypothetical protein	-
MW400_RS04175 (920492)	920492..920635	-	144	Protein_798	HNH endonuclease	-
MW400_RS04180 (920772)	920772..921422	+	651	WP_001037973.1	HNH endonuclease	-
MW400_RS04185 (921430)	921430..922410	-	981	WP_000991420.1	sialate O-acetylesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE / fimA / fimI / fimC	907155..929846	22691

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13064.12 Da
• Isoelectric Point: 9.8743

>T242321 WP_000691792.1 NZ_CP095533:917479-917820 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLTRLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWKEQTPYLRAVDILRARQATGLLRRCHNTTAR

Nucleotide

• Length: 342 bp

>T242321 NZ_OW052570:866440-866733 [Mycobacterium tuberculosis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12582.53 Da
• Isoelectric Point: 7.2122

>AT242321 WP_000939436.1 NZ_CP095533:917113-917448 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKELTCEADTLGSSGYVYIAIYPAQR

Nucleotide

• Length: 336 bp

>AT242321 NZ_OW052570:866174-866443 [Mycobacterium tuberculosis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Structures

Toxin

• AlphaFold DB: V0YJ99

Antitoxin

• AlphaFold DB: V0YMY8