Detailed information of TA system TA242293

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1992326..1993110
• Replicon: chromosome
• Accession: NZ_CP095529
• Organism: Escherichia coli strain GN06156

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: MW397_RS09400
• Protein ID: WP_000613626.1
• Coordinates: 1992326..1992820 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: MW397_RS09405
• Protein ID: WP_001110447.1
• Coordinates: 1992817..1993110 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW397_RS09380 (1987519)	1987519..1988616	+	1098	WP_000589320.1	flagellar basal body P-ring protein FlgI	-
MW397_RS09385 (1988616)	1988616..1989557	+	942	WP_001309406.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
MW397_RS09390 (1989623)	1989623..1991266	+	1644	WP_000096524.1	flagellar hook-associated protein FlgK	-
MW397_RS09395 (1991278)	1991278..1992231	+	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
MW397_RS09400 (1992326)	1992326..1992820	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
MW397_RS09405 (1992817)	1992817..1993110	-	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
MW397_RS09410 (1993243)	1993243..1996428	-	3186	WP_029305445.1	ribonuclease E	-
MW397_RS09415 (1997001)	1997001..1997960	+	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T242293 WP_000613626.1 NZ_CP095529:c1992820-1992326 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T242293 NZ_OW052302:c3573729-3573466 [Mycobacterium tuberculosis]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT242293 WP_001110447.1 NZ_CP095529:c1993110-1992817 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT242293 NZ_OW052302:c3574014-3573733 [Mycobacterium tuberculosis]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6