Detailed information of TA system TA242292

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1745093..1745798
• Replicon: chromosome
• Accession: NZ_CP095529
• Organism: Escherichia coli strain GN06156

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: MW397_RS08230
• Protein ID: WP_000539521.1
• Coordinates: 1745412..1745798 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MW397_RS08225
• Protein ID: WP_001280945.1
• Coordinates: 1745093..1745422 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW397_RS08210 (1740250)	1740250..1741161	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
MW397_RS08215 (1741339)	1741339..1743687	+	2349	WP_001322378.1	EAL domain-containing protein	-
MW397_RS08220 (1743695)	1743695..1745023	+	1329	WP_000086879.1	GGDEF domain-containing protein	-
MW397_RS08225 (1745093)	1745093..1745422	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
MW397_RS08230 (1745412)	1745412..1745798	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MW397_RS08235 (1746024)	1746024..1747349	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
MW397_RS08240 (1747562)	1747562..1747945	+	384	WP_000497137.1	biofilm formation regulator BssR	-
MW397_RS08245 (1748056)	1748056..1749171	+	1116	WP_000555038.1	aldose sugar dehydrogenase YliI	-
MW397_RS08250 (1749168)	1749168..1749794	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T242292 WP_000539521.1 NZ_CP095529:c1745798-1745412 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T242292 NZ_OW052302:c3568169-3567726 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT242292 WP_001280945.1 NZ_CP095529:c1745422-1745093 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT242292 NZ_OW052302:c3568413-3568156 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6