Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 5111028..5111682 Replicon chromosome
Accession NZ_CP095526
Organism Escherichia coli strain GN05505

Toxin (Protein)


Gene name cptA Uniprot ID B7N7E0
Locus tag MW395_RS25750 Protein ID WP_000244769.1
Coordinates 5111275..5111682 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MW395_RS25745 Protein ID WP_000354046.1
Coordinates 5111028..5111294 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW395_RS25725 (5107116) 5107116..5108549 - 1434 WP_001513393.1 6-phospho-beta-glucosidase BglA -
MW395_RS25730 (5108594) 5108594..5108905 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
MW395_RS25735 (5109069) 5109069..5109728 + 660 WP_000250274.1 hemolysin III family protein -
MW395_RS25740 (5109805) 5109805..5110785 - 981 WP_000886049.1 tRNA-modifying protein YgfZ -
MW395_RS25745 (5111028) 5111028..5111294 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MW395_RS25750 (5111275) 5111275..5111682 + 408 WP_000244769.1 protein YgfX Toxin
MW395_RS25755 (5111722) 5111722..5112243 - 522 WP_001055874.1 flavodoxin FldB -
MW395_RS25760 (5112355) 5112355..5113251 + 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
MW395_RS25765 (5113276) 5113276..5113986 + 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MW395_RS25770 (5113992) 5113992..5115725 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16062.03 Da        Isoelectric Point: 11.5554

>T242276 WP_000244769.1 NZ_CP095526:5111275-5111682 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGK
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T242276 NZ_OW052302:c1619738-1619337 [Mycobacterium tuberculosis]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGAGCATGTGCATCATGATGCCGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCCAACGACCACCT
GA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT242276 WP_000354046.1 NZ_CP095526:5111028-5111294 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT242276 NZ_OW052302:c1619962-1619735 [Mycobacterium tuberculosis]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGCCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J3T1

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References