Detailed information of TA system TA242275

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4948749..4949550
• Replicon: chromosome
• Accession: NZ_CP095526
• Organism: Escherichia coli strain GN05505

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: U9ZA48
• Locus tag: MW395_RS24915
• Protein ID: WP_001513431.1
• Coordinates: 4948749..4949126 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: U9YYT7
• Locus tag: MW395_RS24920
• Protein ID: WP_001513429.1
• Coordinates: 4949173..4949550 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW395_RS24890 (4944881)	4944881..4945864	-	984	WP_001329577.1	KpsF/GutQ family sugar-phosphate isomerase	-
MW395_RS24895 (4946674)	4946674..4946805	-	132	Protein_4712	IS110 family transposase	-
MW395_RS24900 (4947187)	4947187..4948029	-	843	WP_001513432.1	DUF4942 domain-containing protein	-
MW395_RS24905 (4948114)	4948114..4948311	-	198	WP_001374283.1	DUF957 domain-containing protein	-
MW395_RS24910 (4948323)	4948323..4948698	-	376	Protein_4715	DUF5983 family protein	-
MW395_RS24915 (4948749)	4948749..4949126	-	378	WP_001513431.1	TA system toxin CbtA family protein	Toxin
MW395_RS24920 (4949173)	4949173..4949550	-	378	WP_001513429.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MW395_RS24925 (4949713)	4949713..4949934	-	222	WP_000692345.1	DUF987 domain-containing protein	-
MW395_RS24930 (4950003)	4950003..4950479	-	477	WP_001186726.1	RadC family protein	-
MW395_RS24935 (4950495)	4950495..4950980	-	486	WP_001513428.1	antirestriction protein	-
MW395_RS24940 (4951035)	4951035..4951853	-	819	WP_001513427.1	DUF932 domain-containing protein	-
MW395_RS24945 (4952032)	4952032..4952121	-	90	WP_112031555.1	DUF905 family protein	-
MW395_RS24950 (4952264)	4952264..4952719	-	456	WP_000581502.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13941.80 Da
• Isoelectric Point: 7.3225

>T242275 WP_001513431.1 NZ_CP095526:c4949126-4948749 [Escherichia coli]
MNTLPDTHVREASGCPSPITIRQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T242275 NZ_OW052302:c1614080-1613685 [Mycobacterium tuberculosis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13688.48 Da
• Isoelectric Point: 5.9505

>AT242275 WP_001513429.1 NZ_CP095526:c4949550-4949173 [Escherichia coli]
VSDTLPGTTPPDDNHDRPWWGLPCTVTSCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Nucleotide

• Length: 378 bp

>AT242275 NZ_OW052302:c1614334-1614080 [Mycobacterium tuberculosis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Structures

Toxin

• AlphaFold DB: U9ZA48

Antitoxin

• AlphaFold DB: U9YYT7