Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3680258..3680755 Replicon chromosome
Accession NZ_CP095526
Organism Escherichia coli strain GN05505

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MW395_RS18890 Protein ID WP_001173343.1
Coordinates 3680258..3680431 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MW395_RS18895 Protein ID WP_000398619.1
Coordinates 3680459..3680755 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW395_RS18880 (3676929) 3676929..3678386 + 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -
MW395_RS18885 (3678623) 3678623..3680140 + 1518 WP_000003806.1 lysine--tRNA ligase -
MW395_RS18890 (3680258) 3680258..3680431 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MW395_RS18895 (3680459) 3680459..3680755 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MW395_RS18900 (3680984) 3680984..3681256 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
MW395_RS18905 (3681268) 3681268..3681498 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
MW395_RS18910 (3681679) 3681679..3683310 + 1632 WP_001216477.1 sensor histidine kinase -
MW395_RS18915 (3683307) 3683307..3684026 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3671405..3680755 9350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T242270 WP_001173343.1 NZ_CP095526:c3680431-3680258 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T242270 NZ_OW052302:1574438-1574875 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT242270 WP_000398619.1 NZ_CP095526:c3680755-3680459 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT242270 NZ_OW052302:1574187-1574441 [Mycobacterium tuberculosis]
ATGAGCATGCGTCTGGCGCATCGGCTCCAGATCCTGCTCGACGACGAGTGCCACCGCCGCATCACCGCGGTGGCGCGCGA
GCGAGGCGTCCCGGTCGCAACCGTCGTGCGTGAGGCCATTGATCGAGGCCTCGTCAGCCCCGCCGGTCGCCGCAAGTCCG
CGGGCAGGCGCCTGCTCGACGCCGCCGACATGTCCGTTCCCGAGCCGCGGGAGCTGAAGCAGGAACTCGAGGCCCTCCGG
GCGCGCCGCGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References