Detailed information of TA system TA242260

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 2315106..2315811
• Replicon: chromosome
• Accession: NZ_CP095526
• Organism: Escherichia coli strain GN05505

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: MW395_RS12240
• Protein ID: WP_000539521.1
• Coordinates: 2315106..2315492 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MW395_RS12245
• Protein ID: WP_001280945.1
• Coordinates: 2315482..2315811 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW395_RS12220 (2311110)	2311110..2311736	+	627	WP_001295292.1	glutathione S-transferase GstB	-
MW395_RS12225 (2311733)	2311733..2312848	-	1116	WP_000555038.1	aldose sugar dehydrogenase YliI	-
MW395_RS12230 (2312959)	2312959..2313342	-	384	WP_000497137.1	biofilm formation regulator BssR	-
MW395_RS12235 (2313555)	2313555..2314880	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
MW395_RS12240 (2315106)	2315106..2315492	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MW395_RS12245 (2315482)	2315482..2315811	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
MW395_RS12250 (2315881)	2315881..2317209	-	1329	WP_000086879.1	GGDEF domain-containing protein	-
MW395_RS12255 (2317217)	2317217..2319565	-	2349	WP_001322378.1	EAL domain-containing protein	-
MW395_RS12260 (2319743)	2319743..2320654	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T242260 WP_000539521.1 NZ_CP095526:2315106-2315492 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T242260 NZ_OW052302:c563104-562700 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT242260 WP_001280945.1 NZ_CP095526:2315482-2315811 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT242260 NZ_OW052302:c563313-563110 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6