Detailed information of TA system TA242258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1614665..1615303
• Replicon: chromosome
• Accession: NZ_CP095526
• Organism: Escherichia coli strain GN05505

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: MW395_RS08455
• Protein ID: WP_000813797.1
• Coordinates: 1615127..1615303 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: MW395_RS08450
• Protein ID: WP_076611057.1
• Coordinates: 1614665..1615081 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW395_RS08430 (1609817)	1609817..1610758	-	942	WP_001251335.1	ABC transporter permease	-
MW395_RS08435 (1610759)	1610759..1611772	-	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
MW395_RS08440 (1611790)	1611790..1612935	-	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
MW395_RS08445 (1613180)	1613180..1614586	-	1407	WP_000760613.1	PLP-dependent aminotransferase family protein	-
MW395_RS08450 (1614665)	1614665..1615081	-	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
MW395_RS08455 (1615127)	1615127..1615303	-	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
MW395_RS08460 (1615525)	1615525..1615755	+	231	WP_000494244.1	YncJ family protein	-
MW395_RS08465 (1615847)	1615847..1617808	-	1962	WP_001309488.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
MW395_RS08470 (1617881)	1617881..1618417	-	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
MW395_RS08475 (1618470)	1618470..1619681	+	1212	WP_001323162.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T242258 WP_000813797.1 NZ_CP095526:c1615303-1615127 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T242258 NZ_OW052302:c360129-359881 [Mycobacterium tuberculosis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATAAGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT242258 WP_076611057.1 NZ_CP095526:c1615081-1614665 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT242258 NZ_OW052302:c360341-360129 [Mycobacterium tuberculosis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A829J715