Detailed information of TA system TA024225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 1299098..1299665
• Replicon: chromosome
• Accession: NC_013165
• Organism: Slackia heliotrinireducens DSM 20476

Toxin (Protein)

• Gene name: relE
• Uniprot ID: C7N5J1
• Locus tag: SHEL_RS05595
• Protein ID: WP_012798279.1
• Coordinates: 1299384..1299665 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C7N5J0
• Locus tag: SHEL_RS05590
• Protein ID: WP_012798278.1
• Coordinates: 1299098..1299376 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SHEL_RS05565	1294695..1295582	+	888	WP_012798273.1	proline iminopeptidase-family hydrolase	-
SHEL_RS05570	1295840..1296331	+	492	WP_012798274.1	RNA polymerase sigma factor	-
SHEL_RS05575	1296328..1297443	+	1116	WP_012798275.1	zf-HC2 domain-containing protein	-
SHEL_RS05580	1297601..1297810	+	210	WP_012798276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
SHEL_RS05585	1297807..1298997	+	1191	WP_012798277.1	glycosyltransferase MGT family	-
SHEL_RS05590	1299098..1299376	+	279	WP_012798278.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
SHEL_RS05595	1299384..1299665	+	282	WP_012798279.1	type II toxin-antitoxin system YafQ family toxin	Toxin
SHEL_RS05600	1299964..1300164	-	201	WP_041422733.1	TM2 domain-containing protein	-
SHEL_RS05605	1300423..1300812	+	390	WP_012798281.1	(deoxy)nucleoside triphosphate pyrophosphohydrolase	-
SHEL_RS05610	1300818..1303742	-	2925	WP_012798282.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-80)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10758.40 Da
• Isoelectric Point: 9.0911

>T24225 WP_012798279.1 NC_013165:1299384-1299665 [Slackia heliotrinireducens DSM 20476]
MSLKLVPTSQFKKDYKRAKKRGLDMRKLQAVLDKLCAEEPLDERHRDHALSGNYIGFRECHVSPDWLLIYAIDKGRLILT
ASRTGTHSDLFDE

Nucleotide

• Length: 282 bp

>T24225 NC_013165:1299384-1299665 [Slackia heliotrinireducens DSM 20476]
ATGTCCTTGAAGCTCGTTCCCACATCGCAGTTCAAGAAGGATTATAAGAGGGCGAAGAAGCGTGGGTTGGACATGCGCAA
GCTCCAGGCTGTGCTTGACAAGCTTTGTGCCGAGGAGCCGCTCGACGAACGCCATCGCGACCATGCGCTATCGGGCAACT
ACATTGGATTTCGTGAATGCCATGTTAGCCCCGATTGGCTGCTGATATACGCGATTGACAAGGGCCGTCTGATACTTACC
GCATCGCGAACAGGGACGCACTCGGATTTGTTCGACGAGTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10017.40 Da
• Isoelectric Point: 4.5680

>AT24225 WP_012798278.1 NC_013165:1299098-1299376 [Slackia heliotrinireducens DSM 20476]
MAKTATINMRVNPEVKSSAETIFASLGMTLTEAINVFLHKSVMEGGLPFDVRQPRYNAETEAAMQEARDIMNGKAETDTF
DSAAAMFAALDK

Nucleotide

• Length: 279 bp

>AT24225 NC_013165:1299098-1299376 [Slackia heliotrinireducens DSM 20476]
ATGGCAAAGACCGCAACAATCAACATGCGCGTGAACCCAGAGGTCAAGTCCAGCGCCGAAACGATCTTCGCAAGCCTGGG
AATGACCCTCACCGAGGCCATCAACGTCTTCTTGCATAAGTCTGTCATGGAGGGCGGGCTCCCCTTCGACGTGCGTCAGC
CCCGTTATAACGCTGAGACGGAAGCTGCCATGCAAGAGGCGCGCGATATCATGAACGGCAAGGCCGAGACGGACACCTTC
GATTCTGCAGCAGCCATGTTTGCGGCGCTCGACAAATAG

Structures

Toxin

• AlphaFold DB: C7N5J1

Antitoxin

• AlphaFold DB: C7N5J0