Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 48241..48766 Replicon plasmid p5505-1
Accession NZ_CP095525
Organism Escherichia coli strain GN05505

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag MW395_RS00350 Protein ID WP_001159871.1
Coordinates 48241..48546 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag MW395_RS00355 Protein ID WP_000813634.1
Coordinates 48548..48766 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW395_RS00315 (43668) 43668..44465 + 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -
MW395_RS00320 (44527) 44527..44778 + 252 Protein_63 DUF4113 domain-containing protein -
MW395_RS00325 (44783) 44783..45175 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
MW395_RS00330 (45180) 45180..46151 - 972 WP_001103694.1 plasmid segregation protein ParM -
MW395_RS00335 (46380) 46380..47024 + 645 WP_000633911.1 AAA family ATPase -
MW395_RS00340 (47018) 47018..47293 + 276 WP_000239529.1 hypothetical protein -
MW395_RS00345 (47431) 47431..48240 - 810 WP_000016979.1 site-specific integrase -
MW395_RS00350 (48241) 48241..48546 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
MW395_RS00355 (48548) 48548..48766 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MW395_RS00360 (51088) 51088..51729 + 642 WP_000990667.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..125360 125360


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T242247 WP_001159871.1 NZ_CP095525:c48546-48241 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T242247 NZ_OW052189:4096402-4096800 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT242247 WP_000813634.1 NZ_CP095525:c48766-48548 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT242247 NZ_OW052189:4096174-4096401 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References