242247

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 48241..48766 Replicon plasmid p5505-1
Accession NZ_CP095525
Organism Escherichia coli strain GN05505

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag MW395_RS00350 Protein ID WP_001159871.1
Coordinates 48241..48546 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag MW395_RS00355 Protein ID WP_000813634.1
Coordinates 48548..48766 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW395_RS00315 (43668) 43668..44465 + 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -
MW395_RS00320 (44527) 44527..44778 + 252 Protein_63 DUF4113 domain-containing protein -
MW395_RS00325 (44783) 44783..45175 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
MW395_RS00330 (45180) 45180..46151 - 972 WP_001103694.1 plasmid segregation protein ParM -
MW395_RS00335 (46380) 46380..47024 + 645 WP_000633911.1 AAA family ATPase -
MW395_RS00340 (47018) 47018..47293 + 276 WP_000239529.1 hypothetical protein -
MW395_RS00345 (47431) 47431..48240 - 810 WP_000016979.1 site-specific integrase -
MW395_RS00350 (48241) 48241..48546 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
MW395_RS00355 (48548) 48548..48766 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MW395_RS00360 (51088) 51088..51729 + 642 WP_000990667.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..125360 125360


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T242247 WP_001159871.1 NZ_CP095525:c48546-48241 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T242247 NZ_OW052189:4096402-4096800 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT242247 WP_000813634.1 NZ_CP095525:c48766-48548 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT242247 NZ_OW052189:4096174-4096401 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References