Detailed information of TA system TA024224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 8472980..8473575
• Replicon: chromosome
• Accession: NC_013131
• Organism: Catenulispora acidiphila DSM 44928

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CACI_RS36085
• Protein ID: WP_190276674.1
• Coordinates: 8472980..8473315 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: C7Q8D3
• Locus tag: CACI_RS36090
• Protein ID: WP_015795849.1
• Coordinates: 8473348..8473575 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CACI_RS36070	8468328..8468699	-	372	WP_041543222.1	DUF4233 domain-containing protein	-
CACI_RS36075	8468699..8470246	-	1548	WP_015795846.1	bifunctional folylpolyglutamate synthase/dihydrofolate synthase	-
CACI_RS36080	8470284..8472932	-	2649	WP_015795847.1	valine--tRNA ligase	-
CACI_RS36085	8472980..8473315	-	336	WP_190276674.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CACI_RS36090	8473348..8473575	-	228	WP_015795849.1	antitoxin	Antitoxin
CACI_RS36095	8473684..8475000	-	1317	WP_015795850.1	ATP-dependent Clp protease ATP-binding subunit ClpX	-
CACI_RS36100	8475216..8475851	-	636	WP_190276842.1	ATP-dependent Clp protease proteolytic subunit	-
CACI_RS36105	8475931..8476557	-	627	WP_015795852.1	ATP-dependent Clp protease proteolytic subunit	-
CACI_RS36110	8476814..8478214	-	1401	WP_015795853.1	trigger factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12087.87 Da
• Isoelectric Point: 10.6629

>T24224 WP_190276674.1 NC_013131:c8473315-8472980 [Catenulispora acidiphila DSM 44928]
MCWVNLDPARGGEIKKRRPAVVVSNDGVNSAVHRYGRGVVTVVPVTSNTERVYPFQILIDAADHPGLRTDSKAQAEQVRT
VDVARIGSRITTLSPSTMRSLDAALRLHLAL

Nucleotide

• Length: 336 bp

>T24224 NC_013131:c8473315-8472980 [Catenulispora acidiphila DSM 44928]
ATGTGCTGGGTCAATCTGGACCCCGCGCGCGGCGGTGAGATCAAGAAACGCCGCCCGGCCGTCGTGGTCTCCAACGACGG
GGTGAACAGCGCCGTCCACCGCTACGGCCGCGGTGTGGTGACCGTCGTGCCCGTCACCTCCAACACTGAGCGCGTGTACC
CCTTCCAGATACTCATCGACGCGGCCGACCATCCGGGTCTGCGCACCGACTCCAAAGCCCAGGCCGAACAGGTCCGCACC
GTGGACGTGGCGCGCATCGGAAGCCGCATCACGACCCTGAGCCCGAGCACGATGCGGAGCCTGGACGCCGCGCTGCGTCT
GCACCTCGCGCTCTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8030.78 Da
• Isoelectric Point: 3.7836

>AT24224 WP_015795849.1 NC_013131:c8473575-8473348 [Catenulispora acidiphila DSM 44928]
MATKLSISLPDADVAFLDELDRNSRSAAVQAAIKLARSVRIVDDYTAAYDEWVSSGEAEVWDGVVGDGIADEADQ

Nucleotide

• Length: 228 bp

>AT24224 NC_013131:c8473575-8473348 [Catenulispora acidiphila DSM 44928]
ATGGCGACGAAGTTGAGCATCTCCCTGCCGGACGCCGATGTGGCCTTCCTGGACGAGTTGGACCGGAACTCCCGCTCGGC
CGCGGTCCAGGCGGCCATCAAGCTCGCCCGGTCGGTGCGCATCGTCGACGACTACACCGCCGCCTACGACGAATGGGTCA
GCTCGGGCGAGGCCGAGGTGTGGGACGGCGTCGTCGGCGACGGGATCGCTGACGAGGCCGACCAGTGA

Structures

Antitoxin

• AlphaFold DB: C7Q8D3