Detailed information of TA system TA242236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 44596..45197
• Replicon: plasmid p4171-3
• Accession: NZ_CP095522
• Organism: Escherichia coli strain GN04171

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: MW404_RS24675
• Protein ID: WP_001216034.1
• Coordinates: 44817..45197 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: MW404_RS24670
• Protein ID: WP_001190712.1
• Coordinates: 44596..44817 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW404_RS24645 (40341)	40341..40646	-	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	-
MW404_RS24650 (40648)	40648..40866	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	-
MW404_RS24655 (41574)	41574..42569	+	996	WP_000246636.1	hypothetical protein	-
MW404_RS24660 (42573)	42573..43505	+	933	WP_000991832.1	S-4TM family putative pore-forming effector	-
MW404_RS24670 (44596)	44596..44817	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MW404_RS24675 (44817)	44817..45197	+	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
MW404_RS24680 (45202)	45202..45381	+	180	WP_001513661.1	hypothetical protein	-
MW404_RS24685 (45409)	45409..45687	+	279	Protein_62	pdcB	-
MW404_RS24690 (45692)	45692..46105	+	414	Protein_63	integrase core domain-containing protein	-
MW404_RS24695 (46055)	46055..46390	-	336	WP_169329198.1	type I deoxyribonuclease HsdR	-
MW404_RS24700 (46600)	46600..47580	-	981	WP_000019407.1	IS5-like element IS5 family transposase	-
MW404_RS24705 (47824)	47824..49227	+	1404	WP_001373486.1	S-methylmethionine permease	-
MW404_RS24710 (49214)	49214..50146	+	933	WP_000081352.1	homocysteine S-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B	-	1..67887	67887
	-	inside	IScluster/Tn	-	-	43642..51297	7655

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T242236 WP_001216034.1 NZ_CP095522:44817-45197 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T242236 NZ_OW052189:c3423283-3422882 [Mycobacterium tuberculosis]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGACCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT242236 WP_001190712.1 NZ_CP095522:44596-44817 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT242236 NZ_OW052189:c3423537-3423280 [Mycobacterium tuberculosis]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6