Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4183038..4183838 Replicon chromosome
Accession NZ_CP095520
Organism Escherichia coli strain GN04171

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag MW404_RS20660 Protein ID WP_000342452.1
Coordinates 4183038..4183565 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag MW404_RS20665 Protein ID WP_001277107.1
Coordinates 4183572..4183838 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW404_RS20635 (4178114) 4178114..4178881 - 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
MW404_RS20640 (4178878) 4178878..4180155 - 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
MW404_RS20645 (4180152) 4180152..4181078 - 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
MW404_RS20650 (4181126) 4181126..4182235 - 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
MW404_RS20655 (4182658) 4182658..4183041 + 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
MW404_RS20660 (4183038) 4183038..4183565 - 528 WP_000342452.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
MW404_RS20665 (4183572) 4183572..4183838 - 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
MW404_RS20670 (4183987) 4183987..4185090 - 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
MW404_RS20675 (4185361) 4185361..4186215 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
MW404_RS20680 (4186460) 4186460..4187518 - 1059 WP_001042013.1 permease-like cell division protein FtsX -
MW404_RS20685 (4187511) 4187511..4188179 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T242230 WP_000342452.1 NZ_CP095520:c4183565-4183038 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T242230 NZ_OW052189:2859261-2859695 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT242230 WP_001277107.1 NZ_CP095520:c4183838-4183572 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT242230 NZ_OW052189:2859076-2859255 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References