Detailed information of TA system TA024223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 2134353..2134921
• Replicon: chromosome
• Accession: NC_013124
• Organism: Acidimicrobium ferrooxidans DSM 10331

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C7M2C8
• Locus tag: AFER_RS10440
• Protein ID: WP_015799393.1
• Coordinates: 2134353..2134691 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AFER_RS10445
• Protein ID: WP_049755579.1
• Coordinates: 2134688..2134921 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFER_RS11840	2129668..2130090	-	423	WP_015799388.1	DUF2256 and DUF3253 domain-containing protein	-
AFER_RS10420	2130454..2130732	+	279	WP_143712044.1	hypothetical protein	-
AFER_RS10425	2130796..2132304	+	1509	WP_015799390.1	cryptochrome/photolyase family protein	-
AFER_RS10430	2133196..2134292	-	1097	Protein_2017	IS3 family transposase	-
AFER_RS10440	2134353..2134691	-	339	WP_015799393.1	PIN domain-containing protein	Toxin
AFER_RS10445	2134688..2134921	-	234	WP_049755579.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
AFER_RS10455	2135403..2135873	-	471	WP_015799395.1	nucleoside deaminase	-
AFER_RS10460	2135860..2136243	-	384	WP_015799396.1	divalent-cation tolerance protein CutA	-
AFER_RS10470	2137295..2137657	-	363	WP_015799397.1	hypothetical protein	-
AFER_RS10475	2137654..2138517	-	864	WP_015799398.1	extradiol ring-cleavage dioxygenase	-
AFER_RS10480	2138539..2139342	-	804	WP_015799399.1	citryl-CoA lyase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2134014..2134292	278

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-107)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 11815.56 Da
• Isoelectric Point: 4.2457

>T24223 WP_015799393.1 NC_013124:c2134691-2134353 [Acidimicrobium ferrooxidans DSM 10331]
MRALLDTSFFVATESGRPLGAMEGVTDTEVSVVTVAELTLGVLMANDDDRPARVATLSAVESTWDPLPVDAEVARQFARV
AAVLHAERRRVPILDVLVAATAMVEGILAGSP

Nucleotide

• Length: 339 bp

>T24223 NC_013124:c2134691-2134353 [Acidimicrobium ferrooxidans DSM 10331]
ATGAGGGCACTGCTCGACACCTCGTTCTTCGTCGCCACTGAGTCGGGTCGACCCCTGGGCGCGATGGAAGGGGTGACCGA
CACAGAGGTGTCGGTGGTGACGGTGGCCGAACTGACGCTCGGCGTGCTCATGGCGAATGACGATGATCGACCGGCGCGAG
TGGCGACGCTTTCAGCCGTGGAGTCGACGTGGGATCCGCTACCGGTGGACGCCGAGGTGGCGCGTCAGTTTGCCCGTGTG
GCCGCCGTGCTGCACGCTGAGCGCCGCCGGGTCCCGATACTCGACGTGCTTGTTGCGGCAACCGCGATGGTCGAAGGGAT
CCTGGCCGGGTCCCCGTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8575.74 Da
• Isoelectric Point: 11.3861

>AT24223 WP_049755579.1 NC_013124:c2134921-2134688 [Acidimicrobium ferrooxidans DSM 10331]
ISVRDLGNTVSEVIRRVESGERLTVTVDRRPVAEIVPLRRRRTVSATEAVAIASRHPADRGLLREVRSLLSDTTDDL

Nucleotide

• Length: 234 bp

>AT24223 NC_013124:c2134921-2134688 [Acidimicrobium ferrooxidans DSM 10331]
ATTTCGGTGCGGGACCTGGGGAACACGGTCAGCGAGGTGATCCGCCGGGTCGAGAGCGGCGAGAGGTTGACGGTCACGGT
TGACCGGCGACCGGTCGCCGAGATCGTGCCCTTGCGCCGTCGCCGGACGGTGTCGGCGACCGAGGCGGTCGCAATTGCGT
CTCGCCACCCGGCCGACCGCGGGCTCCTGAGGGAGGTGCGGAGTCTGCTGTCCGACACGACCGACGATCTATGA

Structures

Toxin

• AlphaFold DB: C7M2C8