Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2625859..2626690 Replicon chromosome
Accession NZ_CP095520
Organism Escherichia coli strain GN04171

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag MW404_RS13165 Protein ID WP_000854815.1
Coordinates 2626316..2626690 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag MW404_RS13160 Protein ID WP_001280918.1
Coordinates 2625859..2626227 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW404_RS13115 (2620948) 2620948..2621694 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
MW404_RS13120 (2621777) 2621777..2622127 + 351 Protein_2574 hypothetical protein -
MW404_RS13125 (2622143) 2622143..2622553 + 411 WP_000846703.1 hypothetical protein -
MW404_RS13130 (2622774) 2622774..2623592 + 819 WP_001542275.1 DUF932 domain-containing protein -
MW404_RS13135 (2623592) 2623592..2623837 + 246 WP_001164966.1 hypothetical protein -
MW404_RS13140 (2623931) 2623931..2624404 + 474 WP_001542276.1 antirestriction protein -
MW404_RS13145 (2624420) 2624420..2624896 + 477 WP_001186200.1 RadC family protein -
MW404_RS13150 (2624959) 2624959..2625180 + 222 WP_000692345.1 DUF987 domain-containing protein -
MW404_RS13155 (2625199) 2625199..2625843 + 645 WP_000086752.1 hypothetical protein -
MW404_RS13160 (2625859) 2625859..2626227 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
MW404_RS13165 (2626316) 2626316..2626690 + 375 WP_000854815.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
MW404_RS13170 (2626687) 2626687..2626881 + 195 WP_000988600.1 DUF5983 family protein -
MW404_RS13175 (2626927) 2626927..2627007 + 81 Protein_2585 hypothetical protein -
MW404_RS13180 (2627296) 2627296..2627376 - 81 WP_023441679.1 hypothetical protein -
MW404_RS13185 (2627355) 2627355..2627678 + 324 WP_225469267.1 EutP/PduV family microcompartment system protein -
MW404_RS13190 (2627779) 2627779..2628108 - 330 WP_000450409.1 DUF496 family protein -
MW404_RS13195 (2628280) 2628280..2629338 - 1059 WP_001200889.1 FUSC family protein -
MW404_RS13200 (2629536) 2629536..2630009 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
MW404_RS13205 (2630128) 2630128..2631294 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T242225 WP_000854815.1 NZ_CP095520:2626316-2626690 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T242225 NZ_OW052189:c2598058-2597675 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT242225 WP_001280918.1 NZ_CP095520:2625859-2626227 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT242225 NZ_OW052189:c2598308-2598153 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References