Detailed information of TA system TA024222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-PHD
• Location: 1709907..1710574
• Replicon: chromosome
• Accession: NC_013124
• Organism: Acidimicrobium ferrooxidans DSM 10331

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C7M0N1
• Locus tag: AFER_RS08390
• Protein ID: WP_015799018.1
• Coordinates: 1709907..1710299 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C7M0N2
• Locus tag: AFER_RS08395
• Protein ID: WP_015799019.1
• Coordinates: 1710299..1710574 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFER_RS08365	1704983..1705735	-	753	WP_015799015.1	GntR family transcriptional regulator	-
AFER_RS08370	1706030..1707220	-	1191	WP_015799016.1	ribonuclease D	-
AFER_RS08375	1707874..1708281	+	408	WP_015799017.1	HIT domain-containing protein	-
AFER_RS08380	1708543..1708743	+	201	WP_083769367.1	transposase	-
AFER_RS11755	1708703..1708924	+	222	WP_143711993.1	hypothetical protein	-
AFER_RS12065	1709134..1709541	+	408	Protein_1618	DDE-type integrase/transposase/recombinase	-
AFER_RS12415	1709594..1709803	+	210	WP_171788920.1	integrase core domain-containing protein	-
AFER_RS08390	1709907..1710299	-	393	WP_015799018.1	PIN domain-containing protein	Toxin
AFER_RS08395	1710299..1710574	-	276	WP_015799019.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
AFER_RS12075	1710666..1710809	-	144	Protein_1622	IS3 family transposase	-
AFER_RS08405	1711139..1711309	+	171	Protein_1623	transposase	-
AFER_RS08410	1711461..1712696	-	1236	WP_015799020.1	tyrosine--tRNA ligase	-
AFER_RS08415	1712709..1714403	-	1695	WP_083769369.1	2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylic-acid synthase	-
AFER_RS11245	1714385..1715221	+	837	WP_015799022.1	alpha/beta fold hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1711124..1711309	185

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-119)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-42)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 13942.85 Da
• Isoelectric Point: 4.4335

>T24222 WP_015799018.1 NC_013124:c1710299-1709907 [Acidimicrobium ferrooxidans DSM 10331]
MVFYLDTSAAAKLVVAEPASEAMASWAASHETQVIASDLLRTELLRATRRAAPDRMQRARSVLDALTLFNLTSATLERAA
TLDPEELRSLDALHLAAALELGDELDGIVTYDNRMAAAASQYGVAVIVPT

Nucleotide

• Length: 393 bp

>T24222 NC_013124:c1710299-1709907 [Acidimicrobium ferrooxidans DSM 10331]
ATGGTCTTCTACCTCGACACGTCGGCGGCGGCCAAGCTCGTTGTCGCCGAACCGGCGTCAGAAGCCATGGCATCGTGGGC
GGCCAGCCACGAAACCCAGGTGATTGCCAGTGACTTGCTGCGCACCGAGCTCCTTCGGGCGACCCGGCGCGCGGCCCCCG
ATCGGATGCAGCGCGCTCGATCAGTACTTGACGCTCTCACCTTGTTCAACCTCACCTCTGCCACGCTCGAACGGGCGGCC
ACCCTTGACCCCGAAGAGTTGCGGAGTCTGGACGCGCTCCATCTTGCCGCAGCTCTGGAACTGGGTGACGAGCTCGACGG
CATCGTCACCTACGACAACCGTATGGCGGCGGCGGCCTCCCAGTACGGCGTCGCGGTGATAGTGCCGACATGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9232.60 Da
• Isoelectric Point: 11.6621

>AT24222 WP_015799019.1 NC_013124:c1710574-1710299 [Acidimicrobium ferrooxidans DSM 10331]
MSTVGIRALKQNASQVVARAAAGEVVTITDRGRPVAQLVPVPEGRVAAFVASGRARPAKGSLAALGAPPDAGAEQPRLSE
VVAAMRGGERY

Nucleotide

• Length: 276 bp

>AT24222 NC_013124:c1710574-1710299 [Acidimicrobium ferrooxidans DSM 10331]
ATGAGTACGGTAGGCATCCGCGCCCTCAAGCAGAACGCCTCGCAGGTGGTGGCCAGAGCCGCCGCCGGCGAGGTGGTGAC
CATCACTGACCGGGGAAGACCGGTCGCCCAGCTCGTTCCGGTCCCGGAGGGGCGAGTCGCTGCCTTCGTCGCGTCGGGTC
GGGCTCGGCCGGCGAAGGGCTCGCTCGCAGCTCTCGGCGCGCCTCCCGACGCGGGTGCGGAGCAGCCCAGGCTGTCGGAG
GTCGTAGCGGCCATGCGTGGCGGGGAGCGCTACTGA

Structures

Toxin

• AlphaFold DB: C7M0N1

Antitoxin

• AlphaFold DB: C7M0N2