Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 896512..897130 Replicon chromosome
Accession NZ_CP095520
Organism Escherichia coli strain GN04171

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MW404_RS04255 Protein ID WP_001291435.1
Coordinates 896512..896730 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MW404_RS04260 Protein ID WP_000344800.1
Coordinates 896756..897130 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW404_RS04220 (891799) 891799..892371 + 573 WP_000779831.1 YbaY family lipoprotein -
MW404_RS04225 (892402) 892402..892713 - 312 WP_000409908.1 MGMT family protein -
MW404_RS04235 (893092) 893092..893445 + 354 WP_000878135.1 DUF1428 family protein -
MW404_RS04240 (893487) 893487..895037 - 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MW404_RS04245 (895201) 895201..895671 - 471 WP_000136192.1 YlaC family protein -
MW404_RS04250 (895787) 895787..896338 - 552 WP_000102539.1 maltose O-acetyltransferase -
MW404_RS04255 (896512) 896512..896730 - 219 WP_001291435.1 HHA domain-containing protein Toxin
MW404_RS04260 (896756) 896756..897130 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MW404_RS04265 (897676) 897676..900825 - 3150 WP_001132478.1 efflux RND transporter permease AcrB -
MW404_RS04270 (900848) 900848..902041 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T242210 WP_001291435.1 NZ_CP095520:c896730-896512 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T242210 NZ_OW052189:c1228784-1228392 [Mycobacterium tuberculosis]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT242210 WP_000344800.1 NZ_CP095520:c897130-896756 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT242210 NZ_OW052189:c1229059-1228784 [Mycobacterium tuberculosis]
ATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCTGCTGCGCCGAGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGCAGGGCGGTCCATACGAGCAGCTGCTGGCAAGCG
GCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGCCCGAACCGCTCGACCTCGACGCGGGCGTCGAGCTGCCGTCG
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References