Detailed information of TA system TA024221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 682871..683477
• Replicon: chromosome
• Accession: NC_013124
• Organism: Acidimicrobium ferrooxidans DSM 10331

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AFER_RS03480
• Protein ID: WP_041662441.1
• Coordinates: 682871..683257 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AFER_RS11530
• Protein ID: WP_083769217.1
• Coordinates: 683250..683477 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFER_RS03445	678436..678918	+	483	WP_015798116.1	transcriptional regulator NrdR	-
AFER_RS03450	678947..679411	+	465	WP_015798117.1	SRPBCC family protein	-
AFER_RS03455	679421..679735	+	315	WP_015798118.1	hypothetical protein	-
AFER_RS03465	680268..680867	+	600	WP_015798119.1	hypothetical protein	-
AFER_RS03470	681103..681972	-	870	WP_012784158.1	IS3 family transposase	-
AFER_RS03475	681972..682292	-	321	WP_012784157.1	transposase	-
AFER_RS03480	682871..683257	-	387	WP_041662441.1	type II toxin-antitoxin system VapC family toxin	Toxin
AFER_RS11530	683250..683477	-	228	WP_083769217.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
AFER_RS03490	683886..684215	-	330	WP_015798121.1	transposase	-
AFER_RS03495	684428..685435	+	1008	WP_015798122.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
AFER_RS03500	685452..686156	+	705	WP_015798123.1	PIG-L family deacetylase	-
AFER_RS10840	686168..686548	+	381	WP_015798124.1	hypothetical protein	-
AFER_RS03510	686663..687019	+	357	WP_015798125.1	DUF3040 domain-containing protein	-
AFER_RS03515	687016..687258	+	243	WP_015798126.1	hypothetical protein	-
AFER_RS11535	687301..687900	+	600	WP_015798127.1	HNH endonuclease	-
AFER_RS03525	687805..688341	-	537	WP_041661666.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (5-120)

Antitoxin

• Post-segregation antitoxin CcdA (3-53)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13852.97 Da
• Isoelectric Point: 6.7617

>T24221 WP_041662441.1 NC_013124:c683257-682871 [Acidimicrobium ferrooxidans DSM 10331]
MAEAVVDASALVDLLLDNELGRAVRRRIAGHALHAPAHVDAEVLSALGRLHRADSLDAGTVEVKLRELAAAPIERHPVHD
LLLGTWARRQELRLVDAIYIELAVSRGVPLVTTDRRLRGAPSVEVVAT

Nucleotide

• Length: 387 bp

>T24221 NC_013124:c683257-682871 [Acidimicrobium ferrooxidans DSM 10331]
ATGGCTGAGGCCGTCGTCGACGCATCCGCTCTGGTCGATCTTCTGCTGGACAATGAGCTCGGTCGTGCGGTGCGGCGTCG
CATCGCTGGTCACGCCTTGCACGCACCGGCGCACGTCGACGCAGAAGTCTTGTCGGCCCTGGGACGCCTGCATCGAGCAG
ACAGCCTCGATGCAGGCACCGTCGAGGTCAAGCTTCGCGAGCTGGCCGCAGCACCGATCGAGCGGCATCCCGTCCACGAT
CTCCTCCTCGGGACCTGGGCCCGTCGCCAAGAGCTTCGGCTGGTCGACGCGATCTATATCGAACTGGCCGTCTCCCGCGG
CGTACCGCTGGTGACGACCGACCGCCGACTCCGCGGTGCACCATCGGTTGAGGTCGTGGCCACCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 7960.96 Da
• Isoelectric Point: 10.3822

>AT24221 WP_083769217.1 NC_013124:c683477-683250 [Acidimicrobium ferrooxidans DSM 10331]
VARVNVYLPDDLAAQARKAGLNLSAVTQEAVRRSLASRSTDAWLATLASPIARVSHERALAALDAARDEAPTRHG

Nucleotide

• Length: 228 bp

>AT24221 NC_013124:c683477-683250 [Acidimicrobium ferrooxidans DSM 10331]
GTGGCTCGAGTGAACGTGTACCTGCCGGACGATCTTGCGGCACAAGCGAGGAAGGCCGGCTTGAATTTGTCTGCGGTGAC
CCAGGAGGCGGTCCGGCGAAGCCTGGCGTCGCGCTCGACAGATGCCTGGCTTGCAACGCTGGCGAGCCCGATTGCGCGAG
TGTCACATGAGCGCGCGCTTGCAGCGCTCGACGCCGCGAGGGACGAAGCACCGACGCGGCATGGCTGA