Detailed information of TA system TA242208

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 445697..446217
• Replicon: chromosome
• Accession: NZ_CP095520
• Organism: Escherichia coli strain GN04171

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A061KX66
• Locus tag: MW404_RS02105
• Protein ID: WP_001160964.1
• Coordinates: 445933..446217 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: MW404_RS02100
• Protein ID: WP_000125566.1
• Coordinates: 445697..445930 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW404_RS02075 (440770)	440770..441057	+	288	WP_000203744.1	ferredoxin-like protein FixX	-
MW404_RS02080 (441116)	441116..442447	+	1332	WP_001183209.1	MFS transporter	-
MW404_RS02085 (442555)	442555..443085	+	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
MW404_RS02090 (443078)	443078..444940	+	1863	WP_000377106.1	glutathione-regulated potassium-efflux system protein KefC	-
MW404_RS02095 (445132)	445132..445611	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
MW404_RS02100 (445697)	445697..445930	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
MW404_RS02105 (445933)	445933..446217	+	285	WP_001160964.1	CcdB family protein	Toxin
MW404_RS02110 (446244)	446244..447092	-	849	WP_000257181.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
MW404_RS02115 (447099)	447099..447476	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
MW404_RS02120 (447479)	447479..448300	-	822	WP_001065365.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
MW404_RS02125 (448297)	448297..449286	-	990	WP_000241227.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
MW404_RS02130 (449286)	449286..450572	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-93)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10692.54 Da
• Isoelectric Point: 10.3030

>T242208 WP_001160964.1 NZ_CP095520:445933-446217 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Nucleotide

• Length: 285 bp

>T242208 NZ_OW052189:c1131482-1131054 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT242208 WP_000125566.1 NZ_CP095520:445697-445930 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT242208 NZ_OW052189:c1131728-1131486 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A061KX66

Antitoxin

• AlphaFold DB: A0A7U9LTM9